Histone H2AT120ph antibody (pAb)

RRID: AB_2744670
商品コード: 39391 Format: 100 µl ¥89,000 Buy
商品コード: 39392 Format: 10 µl ¥18,800 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
16 kDa
Reactivity:
Human, Wide Range Predicted

Applications

Western Blot Validated Dot Blot Validated

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ICC/IF

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This Histone H2A phospho Thr120 antibody was raised against a peptide containing phospho-threonine 120 of histone H2A.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG version (Catalog No. 61195) of this antibody that was purified by Protein A Chromatography is also available.

View our Guide to Histone Modifications and Biological Function.

 
Histone H2AT120ph antibody (pAb) tested by Western blot.

Histone H2A phospho Thr120 pAb tested by Western blot.
HeLa acid extract (20 µg per lane) was probed with Histone H2A phospho Thr120 pAb at a dilution of 1:2,500.
    Lane 1: No treatment.
    Lane 2: Cells treated with colcemid to arrest cells at mitosis.

Histone H2AT120ph antibody (pAb) tested by dot blot analysis.

Histone H2A phospho Thr120 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H2A phospho Thr120 pAb for phospho Thr120 histone H2A. The modified and unmodified peptides to the immunogen were spotted onto PVDF and probed with the antibody at a dilution of 1:2,500. The amount of peptide (picomoles) spotted is indicated next to each row.
    Lane 1: Phospho Thr120 peptide.
    Lane 2: Unmodified Thr120 peptide.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
  • WB = Western Blotting