Histone H2BK46me2 antibody (pAb)

RRID: AB_2793261
商品コード: 39567 Format: 200 µl ¥66,800 Buy
商品コード: 39568 Format: 10 µl ¥18,800 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
15 kDa
Reactivity:
Human, Wide Range Predicted

Applications

Western Blot Validated Immunofluorescence Validated Dot Blot Validated Immunocytochemistry Validated

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This Histone H2B dimethyl Lys46 antibody was raised against a peptide containing dimethyl-lysine 46 of human histone H2B.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

View our Guide to Histone Modifications and Biological Function.

 
Histone H2BK46me2 antibody (pAb) tested by immunofluorescence.

Histone H2B dimethyl Lys46 pAb tested by immunofluorescence.
Top left: HeLa cells stained with Histone H2B dimethyl Lys46 pAb (1:1,000). Top right: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Stained with DAPI. Bottom right: Merge of all 3 images.

Histone H2BK46me2 antibody (pAb) tested by Western blot.

Histone H2B dimethyl Lys46 pAb tested by Western blot.
HeLa acid extract (10 µg per lane) probed with Histone H2B dimethyl Lys46 pAb (1:2,000 dilution).

Histone H2BK46me2 antibody (pAb) tested by dot blot analysis.

Histone H2B dimethyl Lys46 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H2B dimethyl Lys46 pAb for dimethyl Lys46 histone H2B. Methylated peptides corresponding to the immunogen and related sequences derived were spotted onto PVDF and probed with the antibody at 1:20,000. The amount of peptide (picomoles) spotted is indicated next to each row.
    Lane 1: unmodified histone H2B peptide.
    Lane 2: monomethyl Lys46 H2B peptide.
    Lane 3: dimethyl Lys46 H2B peptide.
    Lane 4: trimethyl Lys46 H2B peptide.
    Lane 5: dimethyl Lys4 H3 peptide.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
  • WB = Western Blotting