MeCP2 phospho Ser80 antibody (pAb)

for MeCP2 phospho Ser80 antibody (pAb)Background

Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as development and control of gene expression. It is generally associated with the repressive chromatin state. The complex series of events leading to this repressive state involve the coordinated regulation of DNA methyltransferases and two other groups of proteins called the Methyl-CpG binding proteins (MBD proteins) and the Kaiso family of proteins. The MBD family of proteins include MeCP2, MBD1, MBD2, MBD3 and MBD4. MeCP2 (methyl-CpG binding protein 2)can repress transcription by recruiting the Sin3 complex including histone deacetylases (HDACs). MeCP2 is distributed throughout the nucleus in human cells. Phosphorylation of MeCP2 at serine 80 may regulate the activity of neurons switching between resting and active states in neural circuits.

for MeCP2 phospho Ser80 antibody (pAb)Immunogen

This MeCP2 phospho Ser80 antibody was raised against a peptide containing phospho Ser80 of mouse MeCP2.

for MeCP2 phospho Ser80 antibody (pAb)Application Notes

This MeCP2 phospho Ser80 antibody has been validated for use in Western blotting (1:2,000 - 1:10,000 dilution) and immunohistochemistry. For additional information regarding use of this antibody in IHC, please refer to Tao et al, 2009.

for MeCP2 phospho Ser80 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol, 0.035% sodium azide.

for MeCP2 phospho Ser80 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36059) can be used as a positive control for MeCP2 phospho Ser80 antibody.

for MeCP2 phospho Ser80 antibody (pAb)References

Use of this antibody in IHC is described in Tao, J.et al (2009) PNAS 106: 4882-4887.

for MeCP2 phospho Ser80 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for MeCP2 phospho Ser80 antibody (pAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.