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Antibodies

PAX7 antibody (mAb)

Clone: 2F12H4
Catalog No: 39803 Format: 100 µg $340 Buy Now
Catalog No: 39804 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Rat
Isotype:IgG2b
Purification:Protein G Chromatography
Applications:IF, WB
Reactivity:Mouse
Molecular Weight:55 kDa

Applications Table

Western Blot Chromatin IP Immunofluorescence
0.5 - 2 µg/ml 1 µg/ml

for PAX7 antibody (mAb) (Clone 2F12H4)Background

PAX7 (Paired-box protein 7, PAX7) is a member of the paired box (PAX) family of transcription factors. These proteins are DNA binding transcriptional activators and typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during mammalian development and cancer growth. The specific function of the PAX7 gene is unknown but speculated to play a role in myogenesis. PAX7 is involved in the specification of satellite cells in skeletal muscle. PAX7 can bind to DNA as a heterodimer with Pax-3.

for PAX7 antibody (mAb) (Clone 2F12H4)Immunogen

This PAX7 antibody was raised against a peptide derived from mouse PAX7.

for PAX7 antibody (mAb) (Clone 2F12H4)Application Notes

This PAX7 antibody has been validated for use in immunofluorescence (IF, 1 µg per ml dilution) and Western blot (0.5 - 2 µg per ml dilution).

for PAX7 antibody (mAb) (Clone 2F12H4)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with PAX7 antibody (mAb).

for PAX7 antibody (mAb) (Clone 2F12H4)References

This antibody was used in immunofluorescence and Western blot as described below:
Harada, A. et al (2009) Hybridoma 28: 451-453.

for PAX7 antibody (mAb) (Clone 2F12H4)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for PAX7 antibody (mAb) (Clone 2F12H4)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting
 

Technical Data Sheet

PAX7 antibody (mAb)