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Antibodies

PLZF antibody (mAb)

Clone: 2A9
Catalog No: 39987 Format: 100 µg $340 Buy Now
Catalog No: 39988 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Mouse
Isotype:IgG2a
Purification:Protein G Chromatography
Applications:ChIP, IF, WB
Reactivity:Human
Molecular Weight:90 kDa

Applications Table

Western Blot Chromatin IP Immunofluorescence
0.5 - 2 µg/ml 5 - 10 µg per ChIP 0.5 - 2 µg/ml

for PLZF antibody (mAb) (Clone 2A9)Background

PLZF (Promyelocytic Leukemia Zinc-Finger protein) is a member of the POK (POZ and Krüppel) family of transcriptional repressors. PLZF is expressed in early, but not differentiated, hematopoietic cells, and is required for spermatogonial stem cell maintenance. In addition, PLZF regulates genes involved in cellular proliferation and differentiation (cyclin A2, Myc and Hox genes) and targeted deletion of PLZF in mice results in limb and axial skeleton and limb deformations.

for PLZF antibody (mAb) (Clone 2A9)Immunogen

This PLZF antibody was raised against a recombinant protein corresponding to full length mouse PLZF with a GST epitope tag.

for PLZF antibody (mAb) (Clone 2A9)Application Notes

This PLZF antibody has been validated for use in ELISA, chromatin IP (ChIP, 5 - 10 µg per ChIP), immunofluorescence (0.5 - 2 µg/ml) and Western blotting (0.5 - 2 µg/ml). See references for details.

for PLZF antibody (mAb) (Clone 2A9)Buffer

PBS pH 7.5, 30% glycerol, 0.035% sodium azide

Chromatin IP Assays

Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with PLZF antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use PLZF antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with PLZF antibody (mAb).

for PLZF antibody (mAb) (Clone 2A9)References

This antibody has been used in the following references:
ChIP: Doulatov S et al (2009) Genes Dev 23: 2076-2087.
IF: Koken MH et al (1997) PNAS 94: 10255-10260.
WB: Doulatov S et al (2009) Genes Dev 23: 2076-2087. Shaknovich R et al (1998) Molec Cell Biol 18: 5533-5545.

for PLZF antibody (mAb) (Clone 2A9)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for PLZF antibody (mAb) (Clone 2A9)Guarantee

This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting

Technical Data Sheet

PLZF antibody (mAb)