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NonO antibody (pAb)

Aliases: Non-POU Domain Containing, Octamer-Binding, 54 KDa Nuclear RNA- And DNA-Binding Protein, P54, NMT55, NRB54, P54NRB
商品コード: 61247 Format: 100 µg ¥67,000 Buy Now
商品コード: 61248 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Protein A Chromatography
Host:Rabbit
Molecular Weight:60kD
Reactivity:Human

Applications

Western Blot Validated Immunoprecipitation Validated

for NONO antibody (pAb)Application Notes


Validated Applications:
IP: 5 µg per IP
WB: 0.5 - 2 µg/ml dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for NONO antibody (pAb)Immunogen

This NonO antibody was raised against a peptide corresponding to the C-terminus of human NonO.

for NONO antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for NONO antibody (pAb)References

 
NonO antibody (pAb) tested by immunoprecipitation.

NonO antibody (pAb) tested by Immunoprecipitation.
5 µg of NonO antibody was used to immunoprecipitate NonO from 400 µg of HeLa whole cell extract (lane 2). 5 µg of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the NonO antibody at a dilution of 1 µg/ml.

NonO antibody (pAb) tested by Western blot.

NonO antibody (pAb) tested by Western blot.
Whole cell extract of HeLa cells (20 µg) probed with NonO antibody (1 µg/ml dilution).

for NONO antibody (pAb)Background

NonO (non-POU domain containing, octamer-binding, p54(nrb), NRB54, NMT55) is a DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double-stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. This gene also encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma.

for NONO antibody (pAb)Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for NonO antibody.

for NONO antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for NONO antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

NonO antibody (pAb)
 

Data Thumbnails

NonO antibody (pAb) tested by immunoprecipitation.

Immunoprecipitation of NonO pAb.
(Click image to enlarge and see details.)

NonO antibody (pAb) tested by Western blot.

Western blot of NonO pAb.
(Click image to enlarge and see details.)