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for Recombinant c-Jun proteinContents
5 µg of Recombinant c-Jun protein and Dilution Buffer AM1 (20 mM Tris-Cl (pH 8), 20% glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA). Protein is supplied at 0.4 µg/µl in Dilution Buffer AM1.
for Recombinant c-Jun proteinApplication Notes
Recombinant c-Jun is suitable for DNA and protein-protein interaction assays. 100 ng is sufficient for DNA-protein and protein-protein interaction studies. The molecular weight of the protein is ~43 kDa. The standard curve for TransAM® AP-1 c-Jun was generated using the range of 320-40 ng of protein. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
for Recombinant c-Jun proteinProtein Details
Recombinant c-Jun was expressed from full length (accession number NM 002228) with an amino terminal polyhistidine tag in a baculovirus system and purified by an affinity column in combination with FPLC chromatography. The purified recombinant protein is greater than 95% homogeneous and contains no detectable protease, DNase and RNase activity.
TransAM® standard curve generated using Recombinant c-Jun protein.
The standard curve for TransAM® was generated using a range of 320-40 ng of protein and run on the TransAM® AP-1 c-Jun ELISA Kit.
for Recombinant c-Jun proteinGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
