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Recombinant Histone H3 (C110A)

商品コード: 31207 Format: 100 µg ¥74,000 Buy Now
Expressed In: E. coli Protein Species: Xenopus

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for Recombinant Histone H3 (C110A)Contents

100 µg supplied as lyophilized powder. Recombinant histones can be resuspended in water or any suitable buffer. We recommend a starting concentration of 1 mg/ml. To fully solubilize the histone we suggest resuspension in the buffer of choice at room temperature for 20-30 minutes with occasional pipetting. Addition of salt or Tris to the resuspension buffer may enhance histone solubility.

for Recombinant Histone H3 (C110A)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points. 

Histone H3 (C110A) is a mutant H3 protein that has the single cysteine residue at 110 in the protein mutated to alanine.  This mutant is useful for biophysical studies with assembled nucleosomes, and is useful for certain protein ligation and engineering chemistries used to install site specific modifications into the H3 protein (see Simon, et.al. Cell 2007, and Shimko, et.al. Methods Mol. Biol. 2013).

for Recombinant Histone H3 (C110A)Application Notes

Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatin in vitro.

for Recombinant Histone H3 (C110A)Protein Details

Recombinant Xenopus laevis Histone H3 (C110A) is produced in E. coli and purified using FPLC. Protein concentration was determined using the molar extinction coefficient for Histone H3 and absorbance at 280nm. The recombinant histone is >98% pure by SDS-PAGE. Recombinant Histone H3 C110A contains a substitution of cysteine to alanine at amino acid 110. The molecular weight of the protein is 15,239 Daltons. Please click on the image below to view the mass spectrum.

ESI-TOF mass spectrometry of Recominant Histone H3(C110A)

 
Recombinant Histone H3 (C110A) analyzed by SDS-PAGE gel.

Recombinant Histone H3 (C110A) analyzed by SDS-PAGE gel.
SDS-PAGE analysis of 1.5 µg Recombinant Histone H3 (C110A) (lane 2), Recombinant Histone H3 monomethyl Lys4 (lane 3), Recombinant Histone H3 dimethyl Lys4 (lane 4), and Recombinant Histone H3 trimethyl Lys4 (lane 5). Molecular weight marker is in lane 1.

for Recombinant Histone H3 (C110A)Storage

Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant Histone H3 (C110A)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant Histone H3 (C110A)
 

Data Thumbnails

Recombinant Histone H3 (C110A) analyzed by SDS-PAGE gel.

SDS-PAGE gel of Recombinant Histone H3 (C110A).
(Click image to enlarge and see details.)