Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items

Recombinant HDAC8 protein

Aliases: Histone Deacetylase 8, HD8; WTS; RPD3; CDA07; CDLS5; HDACL1
商品コード: 31353 Format: 50 µg ¥73,000 Buy Now
Expressed In: Baculovirus Protein Species: Human

Request a quote for a bulk orderBulk Order Button


for Recombinant HDAC8 proteinContents

50 µg recombinant HDAC8 supplied in a buffer of 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole and 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant HDAC8 proteinBackground

HDAC8 (Histone Deacetylase 8) is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. May play a role in smooth muscle cell contractility.

for Recombinant HDAC8 proteinApplication Notes

Recombinant HDAC8 is suitable for use in histone deacetylase (HDAC) assays. It can also be used to study enzyme kinetics, inhibitor screening, and selectivity profiling.

Specific Activity: 300 pmol/min/μg.

for Recombinant HDAC8 proteinProtein Details

Full length recombinant human HDAC8 was expressed in a baculovirus expression system (accession number NM_018486) with a C-terminal His-Tag. The molecular weight of the protein is ~46.4 kDa.

 
HDAC8 protein Coomassie gel

HDAC8 protein gel.
HDAC8 run on an SDS-PAGE gel and stained with Coomassie blue.

HDAC8 activity assay

HDA8 activity assay.Recombinant HDAC8 activity using fluorescent HDAC assay.
25mM Tris-HCl, pH 8.0, 137mM NaCl, 2.7mM KCl, 1mM MgCl2, 0.05% Tween 20 and 20 µM HDAC class 2a substrate. Incubate 30 min at 37ºC, followed by incubating with developer at RT for 20 minutes. Fluorescence intensity is measured at exc360/em460.

for Recombinant HDAC8 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant HDAC8 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant HDAC8 protein
 

Data Thumbnails

HDAC8 protein Coomassie gel

HDAC8 protein Coomassie gel.
(Click image to enlarge and see details).

HDAC8 activity assay

HDAC8 activity assay.
(Click image to enlarge and see details).