Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
リコンビナントタンパク質・酵素

Recombinant p300 (1041-1161) protein

Aliases: EP300; KAT3B; RSTS2
商品コード: 31372 Format: 100 µg ¥73,000 Buy Now
商品コード: 31801 Format: 1 mg ¥481,000 Buy Now
Expressed In: E. coli Protein Species: Human

Request a quote for a bulk orderBulk Order Button


for Recombinant p300 (1041-1161) proteinContents

Recombinant p300 (1041-1161) protein expressed in E. coli and supplied in 25 mM Tris pH 7.4, 150 mM NaCl, 5% Glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant p300 (1041-1161) proteinBackground

E1A binding protein p300 (EP300) or p300 regulates cellular growth and differentiation and is also important in preventing tumor growth. Its interaction with adenovirus E1A protein is thought regulate the transforming capacity of E1A. p300 is a transcriptional coactivator with histone acetyl transferase (HAT) activity. It can acetylate all four core histones and regulates transcription via chromatin remodeling. p300 also functions as an acetyltransferase for non-histone targets. Specifically, p300 acetylates 'Lys131' of ALX1 and acts as its coactivator in the presence of CREBBP. p300 is also thought to indirectly increase the transcriptional activity of p53 through acetylation of SIRT2 and subsequent attenuation of its deacetylase function. Additionally, HDAC1 acetylation by p300 leads to HDAC1 inactivation. p300 also acts as a TFAP2A-mediated transcriptional coactivator in the presence of CITED2 and as a coactivator of NEUROD1-dependent transcription of Secretin and p21. Additionally, p300 binds to phosphorylated CREB and mediates cAMP gene regulation. It also regulates terminal differentiation of intestinal epithelial cells. In the case of HIV-1 infection, p300 is recruited by the viral protein TAT and regulates TAT's transactivating activity, and may aid induction of chromatin remodeling of proviral genes. p300 contains a bromodomain that recognizes acetylated histone lysine residues and functions as a ‘reader’ of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets.

for Recombinant p300 (1041-1161) proteinApplication Notes

Recombinant p300 (1041-1161) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

for Recombinant p300 (1041-1161) proteinProtein Details

The peptide corresponding to amino acids 1041 - 1161 that contains the bromodomain sequences of EP300 (accession number NM_001429.3) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with an observed molecular weight of 19.1 kDa. It shows binding specificity for acetylated H3K36, H3K56, H4K12, H4K20 and H4K44, as well as acetylated MyoD-K99/K102. The recombinant protein is >90% pure by SDS-PAGE.

 
p300 (1041-1161) Coomassie gel

Recombinant p300 (1041-1161) protein gel.
p300 (1041-1161) protein was run on a 15% SDS-PAGE gel and stained with Coomassie Blue.

p300 (1041-1161) AlphaScreen<sup>®</sup> assay

Recombinant p300 (1041-1161) activity using AlphaScreen.
p300 (1041-1161) titration and inhibition were assessed using an AlphaScreen® assay. Titration curves were generated to show signal response in the presence of modified peptide substrate at increasing protein concentrations. This data was generated and kindly provided courtesy of ChemPartner.

for Recombinant p300 (1041-1161) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant p300 (1041-1161) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant p300 (1041-1161) protein
 

Data Thumbnails

p300 (1041-1161) Coomassie gel

p300 (1041-1161) Coomassie stained gel.
(Click image to enlarge and see details).

p300 (1041-1161) AlphaScreen<sup>®</sup> assay

p300 (1041-1161) AlphaScreen® assay.
Recombinant p300 (1041-1161) was used in an AlphaScreen® assay to determine enzyme activity and inhibition.
(Click image to enlarge and see details)