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リコンビナントタンパク質・酵素

Recombinant KMT2D (MLL2)-SET protein

Aliases: ALR; KMS; KMT2D; MLL4; AAD10; KABUK1; TNRC21; CAGL114
商品コード: 31420 Format: 50 µg ¥73,000 Buy Now
商品コード: 31820 Format: 1 mg ¥592,000 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant KMT2D (MLL2)-SET proteinContents

Recombinant KMT2D (MLL2)-SET protein was expressed in E. coli cells and supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100.

for Recombinant KMT2D (MLL2)-SET proteinBackground

KMT2D (MLL2)-SET (Myeloid/lymphoid or mixed-lineage leukemia 2) is a Trithorax-group protein that function collectively to promote gene expression. KMT2D (MLL2)-SET is a histone methyltransferase that methylates the Lysine 4 position of histone H3. In particular, the SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. The SET domain is responsible for its histone methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. H3K4 methylation represents a specific epigenetic tag for transcriptional activation. KMT2D (MLL2)-SET is part of a large protein complex called ASCOM, which has been shown to be a transcriptional regulator of the β-globin and estrogen receptor genes. Mutations in this gene have been shown to be a cause of Kabuki syndrome.

for Recombinant KMT2D (MLL2)-SET proteinApplication Notes

Recombinant KMT2D (MLL2)-SET protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

for Recombinant KMT2D (MLL2)-SET proteinProtein Details

The peptide corresponding to amino acids 5355 - 5537 of the KMT2D (MLL2)-SET protein (accession number NP_003473.3) was expressed in E. coli. The peptide contains the SET domain of MLL2 that is responsible for methyltransferase activity. Recombinant KMT2D (MLL2)-SET protein contains an N-terminal GST-Tag with a molecular weight of 47.6 kDa. The recombinant protein is ≥ 80% pure by SDS-PAGE.

 
Recombinant KMT2D (MLL2)-SET protein Coomassie gel.

Recombinant KMT2D (MLL2)-SET protein gel.
KMT2D (MLL2)-SET protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.

Recombinant KMT2D (MLL2)-SET protein binding assay

Recombinant KMT2D (MLL2)-SET protein binding assay.
KMT2D (MLL2)-SET was incubated with equimolar other 4 components of MLL2 complex (ASH2L, RBBP5, WDR5 and DPY30) for 2 hours 4 °C.
All the components were pulled down by GST beads. The sample was run on an 10% SDS-PAGE gel and stained with Coomassie Blue.

for Recombinant KMT2D (MLL2)-SET proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant KMT2D (MLL2)-SET proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant KMT2D (MLL2)-SET protein
 

Data Thumbnails

Recombinant KMT2D (MLL2)-SET protein Coomassie gel.

Recombinant KMT2D (MLL2)-SET protein Coomassie stained gel.
(Click image to enlarge and see details).

Recombinant KMT2D (MLL2)-SET protein binding assay

Recombinant KMT2D (MLL2)-SET protein binding assay.
(Click image to enlarge and see details).