Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items

Recombinant KMT2A (MLL1) complex

商品コード: 31423 Format: 20 µg ¥77,000 Buy Now
商品コード: 31823 Format: 1 mg ¥648,000 Buy Now
Expressed In: E. coli Protein Species: Human

Request a quote for a bulk orderBulk Order Button


for Recombinant KMT2A (MLL1) complexContents

Full length Recombinant KMT2A (MLL1) complex is supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 5% Glycerol and 0.04% Triton X-100. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant KMT2A (MLL1) complexBackground

Recombinant KMT2A (MLL1) complex (myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog 1, Drosophila)), also known as Histone-lysine N-methyltransferase HRX, is a histone methyltransferase that plays an essential role in early development and hematopoiesis. Catalytic subunit of the MLL1 /MLL complex, a multiprotein complex that mediates both methylation of ‘Lys-4’ of histone H3 (H3K4me) complex and acetylation of ‘Lys-16’ of histone H4 (H4K16ac). In the MLL1/MLL complex, it specifically mediates H3K4me, a specific tag for epigenetic transcriptional activation. Has weak methyltransferase activity by itself, and requires other component of the MLL1/MLL complex to obtain full methyltransferase activity. Has no activity toward histone H3 phosphorylated on 'Thr-3', less activity toward H3 dimethylated on 'Arg-8' or 'Lys-9', while it has higher activity toward H3 acetylated on 'Lys-9'. Required for transcriptional activation of HOXA9. Promotes PPP1R15A-induced apoptosis.

for Recombinant KMT2A (MLL1) complexApplication Notes

Recombinant KMT2A (MLL1) complex is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.

Specific Activity: H3K4 methyltransferase

Catalytic Ability: ~300 turnovers/ enzyme molecule

HMT Assay Conditions: 3.3 μM H3K4me0 (1-21aa) peptide was incubated with different concentration of Recombinant KMT2A (MLL1) complex in reaction buffer containing 50 mM TrisCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 100 μM SAM for 3 hours at room temperature. Activity was detected by HTRF and MALDI-TOF.

for Recombinant KMT2A (MLL1) complexProtein Details

Recombinant KMT2A (MLL1) complex contains amino acids 3735 - 3973 of the human MLL1 protein (accession number NP_001184033.1) with N-terminal GST-Tag and MW = 53.7 kDa; full length human WDR5 (GenBank Accession No.NM_017588), with N-terminal 6×His-Tag and MW = 35 kDa; full length human ASH2L (GenBank Accession No. NM_001105214), N-terminal 6×His-Tag and MW = 61 kDa; full length human RbBP5 (GenBank Accession No. NM_005057), N-terminal 6×His-Tag and MW = 60kDa, and full length human DPY30 (GenBank Accession No. NM_032574), N-terminal 6×His-Tag and MW =12 kDa, all individually expressed in E.coli cells. The recombinant protein is >95% pure by SDS-PAGE.

 
Recombinant KMT2A (MLL1) complex Coomassie gel

Recombinant KMT2A (MLL1) complex gel.
Recombinant MLL1 complex was run on an 12% SDS-PAGE gel and stained with Coomassie blue.

Recombinant KMT2A (MLL1) complex HMT activity assay

Recombinant KMT2A (MLL1) complex activity assay.
3.3 uM H3K4me0 peptide was incubated with 10 nM MLL1 complex in reaction buffer for 3 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K4me0 peptide was used as negative control.

Recombinant KMT2A (MLL1) complex HTRF assay

Recombinant KMT2A (MLL1) complex activity assay.
3.3 μM H3K4me0 (1-21aa) peptide was incubated with MLL1 complex in reaction buffer for 3 hour at room temperature. MLL1 complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K4me2) was measured using H3K4me2-specific antibody.

for Recombinant KMT2A (MLL1) complexStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant KMT2A (MLL1) complexGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant KMT2A (MLL1) complex
 

Data Thumbnails

Recombinant KMT2A (MLL1) complex Coomassie gel

Recombinant KMT2A (MLL1) complex Coomassie gel.
(Click image to enlarge and see details).

Recombinant KMT2A (MLL1) complex HMT activity assay

Recombinant KMT2A (MLL1) complex HMT activity assay.
(Click image to enlarge and see details).

Recombinant KMT2A (MLL1) complex HTRF assay

Recombinant KMT2A (MLL1) complex HTRF assay.
(Click image to enlarge and see details).