Sp1 antibody (pAb)

for Sp1 antibody (pAb)Background

Sp1 (specificity protein 1) is a human transcription factor involved in gene expression in the early development of an organism. The protein is 785 amino acids long, with a molecular weight of 81 kDa. The SP1 transcription factor contains a zinc finger protein motif, by which it binds directly to DNA and enhances gene transcription. Its zinc fingers are of the Cys2/His2 type and bind the consensus sequence 5′-(G/T)GGGCGG(G/A)(G/A)(C/T)-3′ (GC box element). It was discovered in 1983 and has since been modified to form Sp1C, which has a zinc finger protein DNA binding domain.

for Sp1 antibody (pAb)Immunogen

This Sp1 antibody was raised against a synthetic peptide corresponding to amino acid residues 520-534 of human Sp1.

for Sp1 antibody (pAb)Application Notes

This Sp1 antibody has been validated for use in Western blotting (1:500 - 1:1,000 dilution) and chromatin IP (5 - 10 µl per ChIP).

For optimal results in Western blot, primary antibody incubations should be performed at 37° C. Individual optimization may be required.

for Sp1 antibody (pAb)Buffer

PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic.

for Sp1 antibody (pAb)Positive Control

Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for Sp1 antibody.

for Sp1 antibody (pAb)References

This antibody was used in ChIP assays as described in Liu, M. et al (2006) Clin Can Res 12(2): 608–616.

for Sp1 antibody (pAb)Storage

Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Sp1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.