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Histone H2A acetyl Lys9 antibody (pAb)
| Catalog No: 39109 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39110 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:2,000 - 1:5,000 | — |
for Histone H2A acetyl Lys9 antibody (pAb)Background
Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H2A and Histone H2B are acetylated in bulk chromatin by p300 and form acetylated H2A/H2B heterodimers. When DNA associates with intact core histone octamers that contain acetylated H2A/H2B heterodimers, the inhibition of transcriptional initiation significantly decreases, indicating that acetylation of their lysine residues may mediate transcription.
for Histone H2A acetyl Lys9 antibody (pAb)Immunogen
This antibody was raised against a peptide including acetyl-lysine 9 of human histone H2A.
for Histone H2A acetyl Lys9 antibody (pAb)Application Notes
This Histone H2A acetyl Lys9 antibody (H2AK9Ac) has been validated for use in Western blotting (1:2,000 - 1:5,000 dilution). Specificity for H2A acetylated at lysine 9 has been confirmed by dot blot and by Western blot against recombinant H2A. This Histone H2A acetyl Lys9 antibody is specific for histone H2A acetylated at lysine 9. Due to evolutionary conservation, a wide range of species reactivity is expected.
for Histone H2A acetyl Lys9 antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
for Histone H2A acetyl Lys9 antibody (pAb)Positive Control
Active Motif's HeLa acid extract (Sodium butyrate treated) (Catalog No. 36202) can be used as a positive control for Histone H2A acetyl Lys9 antibody.
Histone H2A acetyl Lys9 pAb tested by Western blot.
Western blot probed with Histone H2A acetyl Lys9 polyclonal antibody (1:5,000 dilution).
Lane 1: 20 ng recombinant histone H2A.
Lane 2: 5 µg acid extract of HeLa cells.
Lane 3: 5 µg acid extract of HeLa cells treated with sodium butyrate.
Histone H2A acetyl Lys9 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H2A acetyl Lys9 pAb for acetyl Lys9 of Histone H2A. Modified and unmodified peptides were spotted onto PVDF and probed with the antibody at a 1:5,000 dilution. The amount of peptide spotted (in picomoles) is indicated next to each row.
Lane 1: Peptide acetylated at Lys5 of H2A.
Lane 2: Unmodified Lys5 peptide.
Lane 3: Peptide acetylated at Lys9 of H2A.
Lane 4: Unmodified Lys9 peptide.
for Histone H2A acetyl Lys9 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H2A acetyl Lys9 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
