HP1α antibody (pAb)

for HP1 alpha antibody (pAb)Background

HP1 (Heterochromatin protein 1) is a chromosomal protein that participates in both chromatin packaging and gene silencing. Three HP1 isoforms (α, β and γ) occur in mammals. The chromodomain (Chromatin Organization Modifier) of HP1α is responsible for binding to Histone H3 methyl Lys9, although additional factors are required to specify the HP1 targeting. The chromoshadow domain mediates interactions with a number of nuclear proteins, including SuvH39 (a Histone H3 Lysine methyltransferase), the DNA methyltransferases DNMT1 and DNMT3A or the large subunit of the chromatin assembly factor-1 (CAF-1). Dysfunction of HP1α plays a critical role in tumorigenesis, and is implicated in the invasive/metastatic phenotype of breast cancer.

for HP1 alpha antibody (pAb)Immunogen

This HP1 alpha antibody was raised against a recombinant protein corresponding to full-length recombinant mouse HP1 alpha.

for HP1 alpha antibody (pAb)Application Notes

This HP1 alpha antibody has been validated for use in Western blotting (1:500 - 1:1,000 dilution). The antibody recognizes recombinant HP1 alpha, but not HP1 beta or HP1 gamma.

for HP1 alpha antibody (pAb)Buffer

Rabbit serum containing 30% glycerol, 0.035% sodium azide.

for HP1 alpha antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for HP1 alpha antibody.

for HP1 alpha antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for HP1 alpha antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.