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ATM phospho Ser1981 antibody (mAb)
| Clone: 10H11.E.12 |
| Catalog No: 39529 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39530 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| — | — | 1:500 |
for ATM phospho Ser1981 antibody (mAb) (Clone 10H11.E.12)Background
ATM – Ataxia Telangiectasia Mutated is a PI3-family protein kinase and a critical cell cycle checkpoint protein. ATM and a related protein, ATR, play crucial roles in the maintenance of genome integrity and the response to DNA damage. Upon activation, ATM phosphorylates itself at serine 1981 and then phosphorylates a number of downstream proteins (e.g. BRCA1, CHK2, H2AX, p53, NBS1, SMC1) resulting in cell cycle arrest and the initiation of DNA damage repair. Loss of function of ATM is causal to a variety of syndromes involving increased incidence of several cancers.
for ATM phospho Ser1981 antibody (mAb) (Clone 10H11.E.12)Immunogen
This antibody was raised against a peptide containing phospho-serine 1981 of human ATM.
for ATM phospho Ser1981 antibody (mAb) (Clone 10H11.E.12)Application Notes
This ATM phospho Ser1981 antibody (Clone 10H11.E.12) has been validated for use in immunofluorescence (1:500 dilution) on HeLa cells treated with gamma irradiation to cause double strand DNA breaks.
for ATM phospho Ser1981 antibody (mAb) (Clone 10H11.E.12)Buffer
Ascites containing 30% glycerol and 0.035% sodium azide.
for ATM phospho Ser1981 antibody (mAb) (Clone 10H11.E.12)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for ATM phospho Ser1981 antibody (Clone 10H11.E.12).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with ATM phospho Ser1981 antibody (mAb).
for ATM phospho Ser1981 antibody (mAb) (Clone 10H11.E.12)References
This antibody was used in Bakkenist and Kastan (2003) Nature 421:499.
ATM phospho Ser1981 antibody (Clone 10H11.E.12) tested by immunofluorescence.
HeLa cells stained with ATM phospho Ser1981 antibody (Clone 10H11.E.12) at a 1:500 dilution using using MAX Stain™ Immunofluorescence Tools.
Panel A: Normal HeLa cells.
Panel B: HeLa cells treated with 3 Gy ionizing radiation and collected 30 minutes post-exposure.
Top images: Cells stained with DAPI.
Middle images: Same cells stained with ATM phospho Ser1981 mAb (Clone 10H11.E.12).
Bottom images: Merge of the two images above.
Note the Panel B-middle image, which shows intense nuclear clustering of ionizing radiation-induced phosphorylation of ATM at serine 1981. In contrast, Panel A-middle image shows no detectable phosphorylated ATM.
for ATM phospho Ser1981 antibody (mAb) (Clone 10H11.E.12)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for ATM phospho Ser1981 antibody (mAb) (Clone 10H11.E.12)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
