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Ets-1 antibody (pAb)

Aliases: ETS1, EWSR2
商品コード: 39580 Format: 200 µl ¥67,000 Buy Now
商品コード: 39581 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:Serum
Purification:None
Host:Rabbit
Molecular Weight:54 kDa
Reactivity:Human

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated

for Ets-1 antibody (pAb)Application Notes


Validated Applications:
ChIP-Seq: 4 µg (7 µl) per ChIP
WB: 1:500 - 1:1,000 dilution

for Ets-1 antibody (pAb)Immunogen

This Ets-1 antibody was raised against a peptide corresponding to the C-terminal half of mouse Ets-1.

for Ets-1 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Ets-1 antibody (pAb)References

 
ETS-1 antibody (pAb) tested by ChIP-Seq.

ETS-1 antibody (pAb) tested by ChIP-Seq.
Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from human CD4+ T cell chromatin and 4 µg ETS-1 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 7.9 million sequence tags were mapped to identify ETS-1 binding sites in a region of human chromosome 19.

Ets-1 antibody (pAb) tested by Western blot.

ETS-1 pAb tested by Western blot.
Detection of Ets-1 by Western blot. The analysis was performed using CCRF-CEM nuclear extract and ETS-1 pAb at a 1:500 dilution.

for Ets-1 antibody (pAb)Background

Ets-1 transcription factor is a member of the Ets family of proteins that share a unique binding domain. Ets1 (E26 transformation-specific sequence-1) is a proto-oncogene whose DNA binding activity is controlled by kinases and other transcription factors. Ets 1 has been associated with regulation of cell growth, differentiation and apoptosis.

for Ets-1 antibody (pAb)Positive Control

Active Motif's CCRF-CEM nuclear extract (Catalog No. 36006) can be used as a positive control for Ets-1 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Ets-1 antibody (pAb).

for Ets-1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Ets-1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Ets-1 antibody (pAb)
 

Data Thumbnails

ETS-1 antibody (pAb) tested by ChIP-Seq.

ChIP-Seq of ETS-1 pAb.
(Click image to enlarge and see details.)

Ets-1 antibody (pAb) tested by Western blot.

Western blot of ETS-1 pAb.
(Click image to enlarge and see details.)