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PARP-2 antibody (pAb)
| Catalog No: 39743 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 39744 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunoprecipitation |
|---|---|---|
| 1:500 | 2 - 10 µl per ChIP | 2 - 10 µl per IP |
for PARP-2 antibody (pAb)Background
PARP-2 encodes a chromatin-associated enzyme, a poly-ADP ribosyl transferase, which modifies various nuclear proteins by poly-ADP ribosylation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation and tumor transformation. It also plays a role in the regulation of the molecular events involved in the recovery of cells from DNA damage. PARP-2 is cleaved by caspase enzymes during the process of apoptosis.
for PARP-2 antibody (pAb)Immunogen
This PARP-2 antibody was raised against a recombinant protein corresponding to the C-terminal region of human PARP-2.
for PARP-2 antibody (pAb)Application Notes
This PARP-2 antibody (pAb) has been validated for use in Western blotting (1:500 dilution), chromatin immunoprecipitation (ChIP, 2 - 10 µl per ChIP) and immunoprecipitation (2 - 10 µl per IP).
Addition of 0.05% Tween-20 in blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
for PARP-2 antibody (pAb)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with PARP-2 antibody (pAb).
PARP-2 antibody tested by Western blot.
Lane 1: U2OS nuclear extract (20 µg). Lane 2: A549 whole cell extract(20 µg). Lane 3: HepG2 whole cell extract (20 µg). Lane 4: Jurkat cell nuclear extract (20 µg). These were probed with PARP-2 antibody at a dilution of 1:500.
PARP-2 antibody tested by ChIP analysis.
Chromatin IP performed on stably transfected AC16 cells using the per-immune serum (Pre) or the PARP-2 antibody. ChIP DNA was subjected to qPCR using primers specific for human CD36 gene. Data are presented relative to input.
PARP-2 antibody used for immunoprecipitation.
Western blot of immunoprecipitation using the PARP-2 antibody. Lane 1: input (AC16 whole cell lystate). Lane 2: IP using pre-immune serum. Lane 3: IP using PARP-2 antibody.
for PARP-2 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for PARP-2 antibody (pAb)Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
