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Histone H3K18ac antibody (pAb)

商品コード: 39755 Format: 100 µg ¥69,000 Buy Now
商品コード: 39756 Format: 10 µg ¥20,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Protein A Chromatography
Host:Rabbit
Molecular Weight:17 kDa
Reactivity:Human, Other (Wide Range)

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Immunofluorescence Validated Dot Blot Validated Immunocytochemistry Validated

for Histone H3 acetyl Lys18 antibody (pAb)Application Notes


Validated Applications:
ChIP: 10 µg per ChIP
ChIP-Seq: 4 µg each
ICC/IF: 1 µg/ml dilution
WB: 0.5 - 2 µg/ml dilution

Published Applications: see references

for Histone H3 acetyl Lys18 antibody (pAb)Immunogen

This Histone H3 acetyl Lys18 antibody was raised against a peptide including acetyl-lysine 18 of histone H3.

for Histone H3 acetyl Lys18 antibody (pAb)Buffer

Purified IgG in PBS (pH 7.5) with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an unpurified serum version (Catalog No. 39587) of this antibody is also available.

for Histone H3 acetyl Lys18 antibody (pAb)References

 
Histone H3K18ac antibody (pAb) tested by ChIP-chip.

Histone H3 acetyl Lys18 antibody tested by ChIP-chip.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from 2 million Hela cells. ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The image shows a 12 million bp view on chromosome 5 with long stretches of H3K18Ac enrichment

Histone H3K18ac antibody (pAb) tested by ChIP.

Histone H3 acetyl Lys18 antibody tested by ChIP.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 µg of Histone H3 acetyl Lys18 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

Histone H3K18ac antibody (pAb) tested by immunofluorescence.

Histone H3 acetyl Lys18 antibody tested by immunofluorescence.
Detection of Histone H3 acetyl Lys18 by immunofluorescence. HeLa cells were stained with Histone H3 acetyl Lys18 antibody at a dilution of 1 µg/ml. Top panel: Histone H3 acetyl Lys18 antibody staining. Middle panel: DAPI. Bottom panel: merge.

Histone H3K18ac antibody (pAb) tested by Western blot.

.Histone H3 acetyl Lys18 antibody tested by Western blot.
A549 whole-cell extract (20 µg per lane) probed with Histone H3 acetyl Lys18 antibody (0.5 µg/ml).
    Lane 1: Untreated cells.
    Lane 2: Cells treated with Trichostatin A.

Histone H3K18ac antibody (pAb) tested by dot blot analysis.

Histone H3 acetyl Lys18 antibody tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys18 antibody for acetyl Lys18 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 0.1 µg/ml. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: acetyl-Lys4 peptide. Lane 2: unmodified Lys4 peptide. Lane 3: acetyl-Lys18 peptide. Lane 4: unmodified Lys18 peptide. Lane 5: acetyl-Lys9 peptide. Lane 6: acetyl-Lys14 peptide. Lane 7: acetyl-Lys18 peptide 2. Lane 8: acetyl-Lys23 peptide. Lane 9: acetyl-Lys27 peptide.

for Histone H3 acetyl Lys18 antibody (pAb)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Acetylation of histones is linked to a number of specific processes including transcriptional regulation and genomic organization.

for Histone H3 acetyl Lys18 antibody (pAb)Positive Control

Active Motif's HeLa acid extract (Sodium butyrate treated) (Catalog No. 36202) can be used as a positive control for Histone H3 acetyl Lys18 pAb.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K18ac antibody (pAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3K18ac antibody (pAb).

for Histone H3 acetyl Lys18 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3 acetyl Lys18 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H3K18ac antibody (pAb)
 

Data Thumbnails

Histone H3K18ac antibody (pAb) tested by ChIP-chip.

ChIP-chip of Histone H3K18ac pAb.
(Click image to enlarge and see details.)

Histone H3K18ac antibody (pAb) tested by ChIP.

ChIP of Histone H3K18ac pAb.
(Click image to enlarge and see details.)

Histone H3K18ac antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of Histone H3K18ac pAb.
(Click image to enlarge and see details.)

Histone H3K18ac antibody (pAb) tested by Western blot.

Western blot of Histone H3K18ac pAb.
(Click image to enlarge and see details.)

Histone H3K18ac antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H3K18ac pAb.
(Click image to enlarge and see details.)