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Histone H3 acetyl Lys18 antibody (pAb)
| Catalog No: 39755 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 39756 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | ChIP-Seq / ChIP-chip |
IF |
|---|---|---|---|
| 0.1 - 2 µg/ml | 10 µg per ChIP | 4 µg per ChIP-Seq 4 µg per ChIP-chip |
1 µg/ml |
for Histone H3 acetyl Lys18 antibody (pAb)Background
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Acetylation of histones is linked to a number of specific processes including transcriptional regulation and genomic organization.
for Histone H3 acetyl Lys18 antibody (pAb)Immunogen
This Histone H3 acetyl Lys18 antibody was raised against a peptide including acetyl-lysine 18 of histone H3.
for Histone H3 acetyl Lys18 antibody (pAb)Application Notes
This histone H3 acetyl Lys18 antibody (H3K18ac) has been validated for use in chromatin IP (10 µg per ChIP), ChIP-Seq & ChIP-chip (4 µg each), Western blotting (0.1 - 2 µg/ml) and immunofluorescence (1 µg/ml).
for Histone H3 acetyl Lys18 antibody (pAb)Buffer
PBS pH 7.5, 30% glycerol, 0.035% sodium azide.
for Histone H3 acetyl Lys18 antibody (pAb)Positive Control
Active Motif's HeLa acid extract (Sodium butyrate treated) (Catalog No. 36202) can be used as a positive control for Histone H3 acetyl Lys18 pAb.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3 acetyl Lys18 antibody (pAb).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3 acetyl Lys18 antibody (pAb).
Histone H3 acetyl Lys18 antibody tested by Western blot.
A549 whole-cell extract (20 µg per lane) probed with Histone H3 acetyl Lys18 antibody (0.5 µg/ml).
Lane 1: Untreated cells.
Lane 2: Cells treated with Trichostatin A.
Histone H3 acetyl Lys18 antibody tested by ChIP.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 10^6 cell equivalents per ChIP) using 10 µg of Histone H3 acetyl Lys18 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
Histone H3 acetyl Lys18 antibody tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys18 antibody for acetyl Lys18 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 0.1 µg/ml. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: acetyl-Lys4 peptide. Lane 2: unmodified Lys4 peptide. Lane 3: acetyl-Lys18 peptide. Lane 4: unmodified Lys18 peptide. Lane 5: acetyl-Lys9 peptide. Lane 6: acetyl-Lys14 peptide. Lane 7: acetyl-Lys18 peptide 2. Lane 8: acetyl-Lys23 peptide. Lane 9: acetyl-Lys27 peptide.
Histone H3 acetyl Lys18 antibody tested by immunofluorescence.
Detection of Histone H3 acetyl Lys18 by immunofluorescence. HeLa cells were stained with Histone H3 acetyl Lys18 antibody at a dilution of 1 µg/ml. Top panel: Histone H3 acetyl Lys18 antibody staining. Middle panel: DAPI. Bottom panel: merge.
Histone H3 acetyl Lys18 antibody tested by ChIP-chip.
ChIP was performed using chromatin from 2 million Hela cells. ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The image shows a 12 million bp view on chromosome 5 with long stretches of H3K18Ac enrichment.
for Histone H3 acetyl Lys18 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H3 acetyl Lys18 antibody (pAb)Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
