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Androgen Receptor antibody (pAb)
| Catalog No: 39781 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 39782 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | ChIP-Seq / ChIP-chip |
|---|---|---|
| 1 - 2 µg/ml | 5 µg per ChIP | 5 µg per ChIP-Seq 5 µg per ChIP-chip |
for Androgen Receptor antibody (pAb)Background
Androgen Receptor (AR, NR3C4) is a nuclear receptor that facilitates signaling of testosterone and other androgenic hormones in the cytoplasm and translocates into the nucleus upon hormone binding. AR serves as a DNA binding activator of transcription of hormone responsive genes. There are two forms of the Androgen Receptor, the AR-A form, lacking the N-terminal 187 amino acids and the full length AR-B form. Androgen Receptor is involved in the development of both primary and secondary male sexual characteristics and abnormal expression of AR is linked to prostate cancer.
for Androgen Receptor antibody (pAb)Immunogen
This Androgen Receptor antibody was raised against a peptide in the N-terminal region of human Androgen receptor.
for Androgen Receptor antibody (pAb)Application Notes
This Androgen Receptor antibody has been validated for use in chromatin IP (5 µg per ChIP), ChIP-Seq & ChIP-chip (5 µg each) and Western blotting (1 - 2 µg/ml).
for Androgen Receptor antibody (pAb)Buffer
PBS pH 7.5, 30% glycerol, 0.035% sodium azide.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Androgen Receptor antibody (pAb).
Androgen Receptor antibody (pAb) tested by Western blot.
Nuclear extract (20 µg) of LnCaP (Lane 1) and DU145 cells (Lane 2) probed with Androgen Receptor antibody (pAb) (1 µg/ml).
Androgen Receptor antibody (pAb) tested by ChIP.
Chromatin immunoprecipitation (ChIP) was performed using Active Motif's Epigenetic Services protocol with 30 µg of VCAP60 cell chromatin and 10 µg of Androgen receptor antibody. ChIP DNA was used in qPCR with the negative control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.
Androgen Receptor antibody (pAb) tested by ChIP-Seq.
ChIP was performed using chromatin from a human prostate cancer cell line (3 million cells). ChIP DNA was sequenced on the Illumina GAII and 22 million sequence tags were mapped to identify Androgen Receptor binding sites. The image shows hundreds of strong binding sites dispersed across the right arm of human chromosome 16.
for Androgen Receptor antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Androgen Receptor antibody (pAb)Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
