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Antibodies

GCN5 antibody (mAb)

Clone: 2GC-2C11
Catalog No: 39975 Format: 100 µg $340 Buy Now
Catalog No: 39976 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Mouse
Isotype:IgG1
Purification:Protein A Chromatography
Applications:ELISA, IF, WB
Reactivity:Human
Molecular Weight:95 kDa

Applications Table

Western Blot Chromatin IP Immunofluorescence
0.5 - 2 µg/ml 0.5 - 2 µg/ml

for GCN5 antibody (mAb) (Clone 2GC-211)Background

GCN5 (General control of nutrition 5, KAT2A) is a histone acetyltransferase (HAT) and serves as a transcriptional activator. GCN5 is a component of the SAGA and ATAC complexes, which have histone acetyltransferase activity for histones H3 and H4. GCN5 is required for neural tube closure and embryonic development.

for GCN5 antibody (mAb) (Clone 2GC-211)Immunogen

This GCN5 antibody was raised against a synthetic peptide corresponding to amino acids 118-132 of human GCN5.

for GCN5 antibody (mAb) (Clone 2GC-211)Application Notes

This GCN5 antibody has been validated for use in ELISA, immunofluorescence (0.5 - 2 µg/ml), and Western blotting (0.5 - 2 µg/ml). See references for more details.

for GCN5 antibody (mAb) (Clone 2GC-211)Buffer

PBS pH 7.5, 30% glycerol, 0.035% sodium azide

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with GCN5 antibody (mAb).

for GCN5 antibody (mAb) (Clone 2GC-211)References

This antibody has been used in the following references:
IF: Opinell M et al (2010) EMBO J 29: 2381 - 2394.
WB: Brand M et al (2001) EMBO J 20: 3187-3196; Barlev NA et al (2003) Mol Cell Biol 23: 6944-6957.

for GCN5 antibody (mAb) (Clone 2GC-211)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for GCN5 antibody (mAb) (Clone 2GC-211)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting
 

Technical Data Sheet

GCN5 antibody (mAb)