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ATF-6 antibody (mAb)

Clone: 70B1413
Aliases: ATF6A
商品コード: 40962 Format: 100 µg ¥67,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1k
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:90 kDa
Reactivity:Hamster, Human, Mouse, Rat

Applications

ChIP Validated Western Blot Validated Immunohistochemistry Validated Electrophoretic Mobility Shift Assay Validated Flow Cytometry Validated

for ATF-6 antibody (mAb) (Clone 70B1413)Application Notes


Validated Applications:
FC: 1 µg /1x106 cells
IHC: 1:10 - 1:500 dilution
WB*: 1 - 5 µg/ml dilution

Published Applications:
ChIP
WB
EMSA

*For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required. In Western blots the binding pattern of ATF-6 may vary as the antibody may recognize both full-length and cleaved forms of ATF-6. Researchers should consult literature for reported band sizes in their sample or treatment conditions.

for ATF-6 antibody (mAb) (Clone 70B1413)Immunogen

This ATF-6 antibody was raised against a partial protein containing amino acid residues 1-273 of human ATF-6.

for ATF-6 antibody (mAb) (Clone 70B1413)Buffer

PBS containing 0.05% sodium azide. Sodium azide is highly toxic.

for ATF-6 antibody (mAb) (Clone 70B1413)References

 
ATF-6 antibody (mAb) tested by flow cytometry.

ATF-6 antibody (mAb) tested by Flow Cytometry.Intracellular flow cytometric staining of 1x106 MCF-7 cells using ATF6 antibody (dark blue) at a dilution of 1 µg/1x106 cells. Isotype control shown in orange.

ATF-6 antibody (mAb) tested by immunohistochemistry.

ATF-6 antibody (mAb) tested by Immunohistochemistry.ATF6 antibody was used in IHC to stain formalin-fixed, paraffin-embedded human placenta at a 10 µg/ml dilution.

ATF-6 antibody (mAb) tested by Western blot.

ATF-6 antibody (mAb) tested by Western blot.
ATF-6 antibody detection by Western blot. The analysis was performed using 20 µg of whole mouse liver lysate and ATF-6 antibody at a 3 µg/ml dilution. A protein of approximately 90 kDa is detected. The lower band may represent cleaved ATF-6.

for ATF-6 antibody (mAb) (Clone 70B1413)Background

ATF-6 (activating transcription factor 6) is a transmembrane transcription factor that acts during endoplasmic reticulum (ER) stress to activate transcription.

for ATF-6 antibody (mAb) (Clone 70B1413)Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for ATF-6 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with ATF-6 antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use ATF-6 antibody (mAb).

for ATF-6 antibody (mAb) (Clone 70B1413)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for ATF-6 antibody (mAb) (Clone 70B1413)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

ATF-6 antibody (mAb)
 

Data Thumbnails

ATF-6 antibody (mAb) tested by flow cytometry.

Flow Cytometry of ATF-6 mAb.
(Click image to enlarge and see details.)

ATF-6 antibody (mAb) tested by immunohistochemistry.

Immunohistochemistry of ATF-6 mAb.
(Click image to enlarge and see details.)

ATF-6 antibody (mAb) tested by Western blot.

Western blot of ATF-6 mAb.
(Click image to enlarge and see details.)