Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
抗体

RXR-α antibody (mAb)

Clone: 4RX-3A2
Aliases: Rxra, Nr2b1, RXRalpha1
商品コード: 61029 Format: 100 µg ¥67,000 Buy Now

Request a quote for a bulk orderBulk Order Button


Antibody Type:Monoclonal
Isotype:IgG1
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:52 kDa
Reactivity:Human

Applications

Western Blot Validated Immunoprecipitation Validated Electrophoretic Mobility Shift Assay Validated

for RXR-a antibody (mAb) (Clone 4RX-3A2)Application Notes


Published Applications:
IP
EMSA
WB
See references for more information. Individual optimization may be required.

for RXR-a antibody (mAb) (Clone 4RX-3A2)Immunogen

This RXR-alpha antibody was raised against a recombinant protein corresponding to the C-terminal region of mouse RXR-alpha.

for RXR-a antibody (mAb) (Clone 4RX-3A2)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for RXR-a antibody (mAb) (Clone 4RX-3A2)References

for RXR-a antibody (mAb) (Clone 4RX-3A2)Background

RXR-α (Retinoid X Receptor alpha, NR2B1) is a nuclear receptor that mediates the biological effects of retinoids by its involvement in retinoic acid-mediated gene activation. RXR-α exerts its action by binding, as a homodimer or a heterodimer, to specific sequences in the promoters of target genes and regulating their transcription. RXR-α is a member of the steroid and thyroid hormone receptor superfamily of transcriptional regulators.

for RXR-a antibody (mAb) (Clone 4RX-3A2)Positive Control

Active Motif's MCF-7 nuclear extract (Catalog No. 36017) can be used as a positive control for RXR-α antibody.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use RXR-α antibody (mAb).

for RXR-a antibody (mAb) (Clone 4RX-3A2)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for RXR-a antibody (mAb) (Clone 4RX-3A2)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

RXR-α antibody (mAb)