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Antibodies

NR2F2 / COUP2 antibody (pAb)

Catalog No: 61213 Format: 100 µl $340 Buy Now
Catalog No: 61214 Format: 10 µl $70 Buy Now
Antibody Type:Polyclonal
Host:Rabbit
Isotype:IgG
Purification:Affinity Purified
Applications:WB
Reactivity:Human
Molecular Weight:50 kDa

Applications Table

Western Blot Chromatin IP
1:500 - 1:1,000

for NR2F2 / COUP2 antibody (pAb)Background

NR2F2 / COUP2 (ARP1, TFCOUP2, COT2, COUP-TF2) is a member of the nuclear receptor superfamily related to the COUP transcription factor. It interacts with several nuclear receptor co-activator proteins to activate transcription. NR2F2 / COUP2 plays important roles in signaling pathways in multiple endocrine systems and is crucial for differentiation of the trophoblast during development.

for NR2F2 / COUP2 antibody (pAb)Immunogen

This NR2F2 / COUP2 antibody was raised against a peptide within the N-terminal region of human NR2F2.

for NR2F2 / COUP2 antibody (pAb)Application Notes

This NR2F2 / COUP2 antibody has been validated for use in Western blotting (1:500 - 1:1,000 dilution). For optimal results, primary antibody incubations should be performed at 4°C. The addition of 0.1% Tween 20 to all Blotto solutions may reduce background. Individual optimization may be required.

for NR2F2 / COUP2 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

for NR2F2 / COUP2 antibody (pAb)Positive Control

Active Motif's MCF-7 nuclear extract (Catalog No. 36017) can be used as a positive control for NR2F2 / COUP2 antibody.

 
NR2F2 / COUP2 antibody (pAb) tested by Western blot.

NR2F2 / COUP2 (pAb) tested by Western blot.
Detection of NR2F2 / COUP2 by Western blot. The analysis was performed using 40 µg MCF-7 nuclear extract and NR2F2 / COUP2 (pAb) at a 1:500 dilution.

for NR2F2 / COUP2 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for NR2F2 / COUP2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting
 

Technical Data Sheet

NR2F2 / COUP2 antibody (pAb)
 

Data Thumbnails

NR2F2 / COUP2 antibody (pAb) tested by Western blot.

Western blot of NR2F2 / COUP2 pAb.
(Click image to enlarge and see details).