5-Carboxylcytosine (5-caC) antibody (pAb)

for 5-Carboxylcytosine (5-caC) antibody (pAb)Background

DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates.

A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. Iterative activity of TET on 5-hydroxymethylcytosine results in the conversion of 5-hmC into 5-formylcytosine and 5-carboxylcytosine. An increase in levels of 5-formylcytosine and 5-carboxylcytosine are detected in the mouse male pronucleus following fertilization, which is gradually diluted by DNA replication.

for 5-Carboxylcytosine (5-caC) antibody (pAb)Immunogen

This 5-Carboxylcytosine antibody was raised against 5-carboxylcytidine conjugated to KLH and recognizes 5-carboxylcytosine.

for 5-Carboxylcytosine (5-caC) antibody (pAb)Application Notes

This antibody recognizing 5-carboxylcytosine has been validated for use in dot blot (0.5 - 1 µg/ml).

for 5-Carboxylcytosine (5-caC) antibody (pAb)Buffer

Purified IgG in PBS, 30% glycerol, 0.035% sodium azide.

for 5-Carboxylcytosine (5-caC) antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for 5-Carboxylcytosine (5-caC) antibody (pAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.