EZH2 phospho Thr345 antibody (pAb)

for EZH2 phospho Thr345 antibody (pAb)Background

EZH2 – (Enhancer of Zeste homolog 2, also designated Enx1) is a human homolog of the Drosophila Polycomb-group protein Enhancer of Zeste protein. It contains a SET domain that catalyzes the methylation of histone H3 at lysine 27 (Histone H3 Lys27 monomethylated, dimethylated and trimethylated). Polycomb-group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. EZH2, BMI-1 and Suz12 are present in the PRC2 and PRC3 protein complexes that function as mediators of epigenetic transcriptional silencing. Threonine 345 of mouse Ezh2 is phosphorylated in a cell cycle-dependent manner and may play a role in the interaction of Ezh2 with HOTAIR and the 59-RepA repeats of Xist. (see reference)

for EZH2 phospho Thr345 antibody (pAb)Immunogen

This EZH2 phospho Thr345 antibody was raised against a peptide containing phosphothreonine 345 of mouse EZH2 (enhancer of zeste homolog 2 (Drosophila))

for EZH2 phospho Thr345 antibody (pAb)Application Notes

This EZH2 phospho Thr345 antibody has been validated for use in Western blotting. See references for more details.

for EZH2 phospho Thr345 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

for EZH2 phospho Thr345 antibody (pAb)References

WB: Kaneko S, et al (2010) 24(23):2615-2620　

for EZH2 phospho Thr345 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for EZH2 phospho Thr345 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of arrival.