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SMAD3 antibody (pAb)
| Catalog No: 61249 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 61250 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:1,000 | — |
for SMAD3 antibody (pAb)Background
This SMAD3 (mothers against decapentaplegic homolog 3, mad3, MAD homolog 3) protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
for SMAD3 antibody (pAb)Immunogen
This SMAD3 antibody was raised against a peptide within human SMAD3 (mothers against decapentaplegic homolog 3).
for SMAD3 antibody (pAb)Application Notes
This SMAD3 antibody has been validated for use in Western blot (1:500 - 1:1,000).
Addition of 0.05% Tween-20 in blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
for SMAD3 antibody (pAb)Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.
SMAD3 antibody (pAb) tested by Western blot.
Whole cell extract of A549 cells (30 µg) probed with SMAD3 antibody (1:500).
for SMAD3 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for SMAD3 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
