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SMAD3 antibody (pAb)

Aliases: LDS3, LDS1C, MADH3, JV15-2, HSPC193, HsT17436
商品コード: 61249 Format: 100 µl ¥67,000 Buy Now
商品コード: 61250 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:52kD
Reactivity:Human

Applications

Western Blot Validated

for SMAD3 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for SMAD3 antibody (pAb)Immunogen

This SMAD3 antibody was raised against a peptide within human SMAD3 (mothers against decapentaplegic homolog 3).

for SMAD3 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for SMAD3 antibody (pAb)References

 
SMAD3 antibody (pAb) tested by Western blot.

SMAD3 antibody (pAb) tested by Western blot.
Whole cell extract of A549 cells (30 µg) probed with SMAD3 antibody (1:500).

for SMAD3 antibody (pAb)Background

This SMAD3 (mothers against decapentaplegic homolog 3, mad3, MAD homolog 3) protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

for SMAD3 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for SMAD3 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

SMAD3 antibody (pAb)
 

Data Thumbnails

SMAD3 antibody (pAb) tested by Western blot.

Western blot of SMAD3 pAb.
(Click image to enlarge and see details.)