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TAZ / WWTR1 antibody (pAb)

商品コード: 61265 Format: 100 µl ¥67,000 Buy Now
商品コード: 61266 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:55 kDa
Reactivity:Human

Applications

Western Blot Validated

for TAZ / WWTR1 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

for TAZ / WWTR1 antibody (pAb)Immunogen

TAZ / WWTR1 antibody was raised against a peptide corresponding to the C-terminal region of human TAZ / WWTR1.

for TAZ / WWTR1 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for TAZ / WWTR1 antibody (pAb)References

 
TAZ / WWTR1 antibody (pAb) tested by Western blot.

TAZ / WWTR1 antibody (pAb) tested by Western blot
Nuclear extract of A-431 cells (30 µg) probed with TAZ / WWTR1 antibody (1:500).

for TAZ / WWTR1 antibody (pAb)Background

The TAZ / WWTR1 (Transcriptional coactivator with PDZ-binding motif, WW domain-containing transcription regulator protein 1) gene encodes a protein that is a transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition.

for TAZ / WWTR1 antibody (pAb)Positive Control

Active Motif's A-431 nuclear extract (Catalog No. 36004) can be used as a positive control for TAZ / WWTR1 antibody.

for TAZ / WWTR1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for TAZ / WWTR1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

TAZ / WWTR1 antibody (pAb)
 

Data Thumbnails

TAZ / WWTR1 antibody (pAb) tested by Western blot.

Western blot of TAZ / WWTR1 pAb.
(Click image to enlarge and see details.)