Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
抗体

Sf3b3 antibody (mAb)

Clone: 1YO-1B10
Aliases: Splicing Factor 3b Subunit 3, RSE1, SAP130, SF3b130, STAF130
商品コード: 61345 Format: 100 µg ¥67,000 Buy Now
商品コード: 61346 Format: 10 µg ¥19,000 Buy Now

Request a quote for a bulk orderBulk Order Button


Antibody Type:Monoclonal
Isotype:IgG1k
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:135 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated Immunoprecipitation Validated

for Sf3b3 antibody (mAb) (Clone 1YO-1B10)Application Notes


Published Applications:
IP
WB
See references for more information. Individual optimization may be required.

for Sf3b3 antibody (mAb) (Clone 1YO-1B10)Immunogen

This antibody was raised against a peptide corresponding to amino acid residue(s) 1057-1076 of human Sf3b3.

for Sf3b3 antibody (mAb) (Clone 1YO-1B10)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for Sf3b3 antibody (mAb) (Clone 1YO-1B10)References

for Sf3b3 antibody (mAb) (Clone 1YO-1B10)Background

Sf3b3 (splicing factor 3b, subunit 3, 130kDa) is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Sf3b3 antibody (mAb).

for Sf3b3 antibody (mAb) (Clone 1YO-1B10)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Sf3b3 antibody (mAb) (Clone 1YO-1B10)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Sf3b3 antibody (mAb)