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Ddit3 antibody (mAb)

Clone: 9C8
Aliases: CHOP, CEBPZ, CHOP10, CHOP-10, GADD153
商品コード: 61349 Format: 100 µg ¥67,000 Buy Now
商品コード: 61350 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2b
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:29 kDa
Reactivity:Mouse

Applications

Western Blot Validated Immunoprecipitation Validated

for Ddit3 antibody (mAb) (Clone 9C8)Application Notes


Published Applications:
IP
WB
See references for more information. Individual optimization may be required.

for Ddit3 antibody (mAb) (Clone 9C8)Immunogen

This antibody was raised against full-length recombinant mouse Ddit3 protein.

for Ddit3 antibody (mAb) (Clone 9C8)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

for Ddit3 antibody (mAb) (Clone 9C8)References

for Ddit3 antibody (mAb) (Clone 9C8)Background

Ddit3 (DNA-damage-inducible transcript 3, CHOP) is a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Ddit3 antibody (mAb).

for Ddit3 antibody (mAb) (Clone 9C8)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Ddit3 antibody (mAb) (Clone 9C8)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Ddit3 antibody (mAb)