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ATAD2 antibody (pAb)

Aliases: ANCCA, CT137, PRO2000
商品コード: 61369 Format: 100 µl ¥67,000 Buy Now
商品コード: 61370 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:160 kDa
Reactivity:Human

Applications

Western Blot Validated Immunoprecipitation Validated

for ATAD2 antibody (pAb)Application Notes


Validated Applications:
IP: 5 - 10 µl per IP
WB: 1:500 - 1:2,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for ATAD2 antibody (pAb)Immunogen

This antibody was raised against a peptide within the C-terminal region of human ATAD2.

for ATAD2 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

for ATAD2 antibody (pAb)References

 
ATAD2 antibody (pAb) tested by immunoprecipitation.

ATAD2 antibody (pAb) tested by Immunoprecipitation.
10 µl of ATAD2 antibody was used to immunoprecipitate ATAD2 from 250 µg of Jurkat nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the ATAD2 antibody at a dilution of 1:1,000.

ATAD2 antibody (pAb) tested by Western blot.

ATAD2 antibody (pAb) tested by Western blot.
ATAD2 detection by Western blot. The analysis was performed using 40 µg Jurkat nuclear cell extract and CENP-B at a 1:1,000 dilution.

for ATAD2 antibody (pAb)Background

ATAD2 (ATPase family AAA domain-containing protein 2) may be a transcriptional coactivator of the nuclear receptor ESR1 required to induce the expression of a subset of estradiol target genes, such as CCND1, MYC and E2F1. May play a role in the recruitment or occupancy of CREBBP at some ESR1 target gene promoters. May be required for histone hyperacetylation. Involved in the estrogen-induced cell proliferation and cell cycle progression of breast cancer cells.

for ATAD2 antibody (pAb)Positive Control

Active Motif's Jurkat nuclear extract (Catalog No. 36014) can be used as a positive control for ATAD2 antibody.

for ATAD2 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for ATAD2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

ATAD2 antibody (pAb)
 

Data Thumbnails

ATAD2 antibody (pAb) tested by immunoprecipitation.

Immunoprecipitation of ATAD2 pAb.
(Click image to enlarge and see details.)

ATAD2 antibody (pAb) tested by Western blot.

Western blot of ATAD2 pAb.
(Click image to enlarge and see details.)