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Histone H3K9me0 antibody (mAb)

Clone: 9B1-2G6
商品コード: 61399 Format: 100 µg ¥67,000 Buy Now
商品コード: 61400 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:17 kDa
Reactivity:Human, Other (Wide Range)

Applications

Western Blot Validated

for Histone H3 unmethyl Lys9 antibody (mAb) (Clone 9B1-2G6)Application Notes


Validated Applications:
WB: 0.5 - 2 µg/ml dilution

for Histone H3 unmethyl Lys9 antibody (mAb) (Clone 9B1-2G6)Immunogen

This antibody was raised against a synthetic branched peptide corresponding to amino acids surrounding Lys9 of human Histone H3.

for Histone H3 unmethyl Lys9 antibody (mAb) (Clone 9B1-2G6)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

Also available as an AbFlex™ engineered recombinant antibody.  For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91155.

for Histone H3 unmethyl Lys9 antibody (mAb) (Clone 9B1-2G6)References

 
Histone H3K9me0 antibody (mAb) tested by Western blot.

Histone H3K9me0 mAb tested by Western blot.
Histone H3K9me0 detection by Western blot. The analysis was performed using HeLa acid extract.

Histone H3K9me0 antibody (mAb) tested by dot blot analysis.

Histone H3K9me0 mAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3K9me0 mAb for detection of unmethylated Lys9 of Histone H3. 50 pmol of recombinant Histone H3 proteins containing various modifications were spotted onto PVDF and probed with the antibody at 1 µg/mL.
    Lane 1: Histone H3 unmodified.
    Lane 2: Histone H3K4me3.
    Lane 3: Histone H3K9me3.
    Lane 4: Histone H3 K9ac.
    Lane 5: Histone H3 K14ac.
    Lane 6: Histone H3K4ac.
    Lane 7: Histone H3 S10ph.

for Histone H3 unmethyl Lys9 antibody (mAb) (Clone 9B1-2G6)Background

Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Methylation of lysines occur as four states: unmethylated (me0), monomethyl (me1), dimethyl (me2) and trimethyl (me3). The me0 state of lysine is recognized as biologically relevant and a number of proteins containing PhD fingers, ADD and WD40 domains are known to associate with unmodified lysines.

for Histone H3 unmethyl Lys9 antibody (mAb) (Clone 9B1-2G6)Positive Control

Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H3K9me0 antibody.

for Histone H3 unmethyl Lys9 antibody (mAb) (Clone 9B1-2G6)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3 unmethyl Lys9 antibody (mAb) (Clone 9B1-2G6)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H3K9me0 antibody (mAb)
 

Data Thumbnails

Histone H3K9me0 antibody (mAb) tested by Western blot.

Western blot of Histone H3K9me0 mAb.
(Click image to enlarge and see details.)

Histone H3K9me0 antibody (mAb) tested by dot blot analysis.

Dot blot of Histone H3K9me0 mAb.
(Click image to enlarge and see details.)