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Supt16H / Spt16 antibody (pAb)

Aliases: CDC68, FACTP140, SPT16/CDC68
商品コード: 61441 Format: 100 µl ¥67,000 Buy Now
商品コード: 61442 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:140 kDa
Reactivity:Human

Applications

Western Blot Validated

for Supt16H / Spt16 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for Supt16H / Spt16 antibody (pAb)Immunogen

This antibody was raised against a synthetic peptide containing human Supt16H / Spt16.

for Supt16H / Spt16 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Supt16H / Spt16 antibody (pAb)References

 
Supt16H / Spt16 antibody (pAb) tested by Western blot.

Supt16H / Spt16 (pAb) tested by Western blot.
Supt16H / Spt16 detection by Western blot. The analysis of Supt16H / Spt16 was performed using K-562 nuclear extract (30 μg) and Supt16H / Spt16 (pAb) at a 1:500 dilution.

for Supt16H / Spt16 antibody (pAb)Background

Supt16H / Supt16 (suppressor of Ty 16 homolog (S. cerevisiae)) is a component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene (By similarity).

for Supt16H / Spt16 antibody (pAb)Positive Control

Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for Supt16H / Spt16 antibody.

for Supt16H / Spt16 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Supt16H / Spt16 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Supt16H / Spt16 antibody (pAb)
 

Data Thumbnails

Supt16H / Spt16 antibody (pAb) tested by Western blot.

Western blot of Supt16H / Spt16 pAb.
(Click image to enlarge and see details.)