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Histone H3 antibody (mAb)

Clone: 1B1-B2
商品コード: 61475 Format: 100 µg ¥67,000 Buy Now
商品コード: 61476 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG3
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:17 kDa
Reactivity:Human, Other (Wide Range)

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated

for Histone H3 antibody (mAb) (Clone 1B1-B2)Application Notes


Validated Applications:
ChIP-Seq: 4 µg per ChIP
ChIP: 5 µg per ChIP
WB: 1 - 4 µg/ml dilution

Published Applications:
WB

for Histone H3 antibody (mAb) (Clone 1B1-B2)Immunogen

This antibody was raised against a peptide within the C-terminal region of human histone H3.

for Histone H3 antibody (mAb) (Clone 1B1-B2)Buffer

Purified IgG in 100mM sodium phosphate pH 8.0, 90mM trisodium citrate, 430mM sodium chloride.
If crystals are noted, warm to room temperature and mix gently before use.

for Histone H3 antibody (mAb) (Clone 1B1-B2)References

 
Histone H3 antibody (mAb) tested by ChIP-Seq.

Histone H3 antibody (mAb) tested by ChIP-Seq.Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from Farage human lymph-node derived B cells and 5 µl Histone H3 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 16.7 million sequence tags were mapped to identify Histone H3 binding sites.

Histone H3 antibody (mAb) tested by ChIP.

H3 antibody (mAb) tested by ChIP.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 15 µg of chromatin from both HeLa and K562 cells and 4 µg H3 antibody. ChIP DNA was used in qPCR with the gene-specific primer as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

Histone H3 antibody (mAb) tested by Western blot.

Histone H3 antibody (mAb) (Clone 1B1-B2) tested by Western blot.
Histone H3 antibody detection by Western blot. The analysis was performed using 20 µg of HeLa nuclear extract and Histone H3 antibody at a 0.25 µg/ml dilution.

for Histone H3 antibody (mAb) (Clone 1B1-B2)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

for Histone H3 antibody (mAb) (Clone 1B1-B2)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone H3 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3 antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Histone H3 antibody (mAb).

for Histone H3 antibody (mAb) (Clone 1B1-B2)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3 antibody (mAb) (Clone 1B1-B2)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H3 antibody (mAb)
 

Data Thumbnails

Histone H3 antibody (mAb) tested by ChIP-Seq.

ChIP-Seq of Histone H3 mAb.
(Click image to enlarge and see details.)

Histone H3 antibody (mAb) tested by ChIP.

ChIP of Histone H3 mAb.
(Click image to enlarge and see details.)

Histone H3 antibody (mAb) tested by Western blot.

Western blot of Histone H3 mAb.
(Click image to enlarge and see details.)