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N6-Methyladenosine (m6A) antibody (pAb)

商品コード: 61495 Format: 100 µg ¥67,000 Buy Now
商品コード: 61496 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Protein A Chromatography
Host:Rabbit
Molecular Weight:Not Applicable
Reactivity:Other (Not Species Specific)

Applications

Dot Blot Validated

for N6-Methyladenosine (m6A) antibody (pAb)Application Notes


Validated Applications:
DB: 1 - 2 µg/ml dilution

for N6-Methyladenosine (m6A) antibody (pAb)Immunogen

This antibody was raised against 6-Methyladenosine conjugated to KLH.

for N6-Methyladenosine (m6A) antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for N6-Methyladenosine (m6A) antibody (pAb)References

 
N6-Methyladenosine (m6A) antibody (pAb) tested by dot blot analysis.

N6-Methyladenosine (m6A) antibody (pAb) tested by RNA dot blot analysis.
3.3ng of a 22 nt RNA containing four repeats of the GAC consensus for N6-methyladenosine within its sequence was spotted onto a membrane and probed with N6-Methyladenosine antibody (1 µg/ml). The sequence contained various adenosine modifications at all four positions as indicated.
Lane 1: Adenosine.
Lane 2: N6-methyladenosine.
Lane 3: 1-methyladenosine.
Lane 4: 2’-O-methyladenosine.
Lane 5: inosine

N6-Methyladenosine (m6A) antibody (pAb) tested by dot blot analysis.

N6-Methyladenosine (m6A) antibody (pAb) tested by DNA dot blot analysis.
Single-stranded DNA oligonucleotides (amount of oligo in pmoles listed on the left side of the blot) were spotted on to a positively charged nylon membrane and blotted with N-6-methyladenosine antibody (1 µg/ml dilution).
Lane 1: DNA containing a single 6-methyladenosine.
Lane 2: DNA containing unmethylated adenosine.
Lane 3: DNA containing a single 1-methyladenosine.

for N6-Methyladenosine (m6A) antibody (pAb)Background

N6-Methyladenosine (m6A) ) is an RNA modification on the N-6 position of adenosine. This modification has been found to be abundant in the 3’ UTR and stop codons of mammalian mRNA. m6A is associated with miRNA binding sites suggesting a potential role in epigenetic gene regulation. FTO and ALKBH are demethylases for 6-methyladenosine while a multiprotein complex that includes METTL3 functions as the methyltransferase. Recent findings revealed that m6A is also present on metazoan DNA, suggesting a genuine epigenetic role for this modification in the context of DNA as well.

for N6-Methyladenosine (m6A) antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for N6-Methyladenosine (m6A) antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

N6-Methyladenosine (m6A) antibody (pAb)
 

Data Thumbnails

N6-Methyladenosine (m6A) antibody (pAb) tested by dot blot analysis.

RNA Dot blot of N6-Methyladenosine (m6A) pAb.
(Click image to enlarge and see details.)

N6-Methyladenosine (m6A) antibody (pAb) tested by dot blot analysis.

DNA Dot blot of N6-Methyladenosine (m6A) pAb.
(Click image to enlarge and see details.)