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BRPF1 antibody (pAb)

Aliases: BR140
商品コード: 61541 Format: 100 µl ¥67,000 Buy Now
商品コード: 61542 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:150 kDa
Reactivity:Mouse

Applications

Western Blot Validated

for BRPF1 antibody (pAb)Application Notes


Validated Applications:
WB: 1:1,000 - 1:2,500 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for BRPF1 antibody (pAb)Immunogen

This antibody was raised against a peptide within the C-terminal region of mouse BRPF1.

for BRPF1 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for BRPF1 antibody (pAb)References

 
BRPF1 antibody (pAb) tested by Western blot.

BRPF1 antibody (pAb) tested by Western blot.
BRPF1 detection by Western blot. The analysis was performed using 20 µg mouse testis nuclear cell extract and BRPF1 antibody at a 1:1,000 dilution.

for BRPF1 antibody (pAb)Background

BRPF1 (bromodomain and PHD finger containing, 1) is a component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. The bromodomain recognizes acetylated histones and the PWWP domain has been reported to interact with trimethylated Lys36 of Histone H3 (H3K36me3). Positively regulates the transcription of RUNX1 and RUNX2.

for BRPF1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for BRPF1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

BRPF1 antibody (pAb)
 

Data Thumbnails

BRPF1 antibody (pAb) tested by Western blot.

Western blot of BRPF1 pAb.
(Click image to enlarge and see details.)