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MED15 antibody (pAb)

Aliases: TIG1, CAG7A, CTG7A, PCQAP, TIG-1, TNRC7, ARC105
商品コード: 61589 Format: 100 µl ¥67,000 Buy Now
商品コード: 61590 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:100 kDa
Reactivity:Human

Applications

Western Blot Validated Immunoprecipitation Validated

for MED15 antibody (pAb)Application Notes


Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:2,500 dilution

for MED15 antibody (pAb)Immunogen

This antibody was raised against a peptide within the C-terminal region of human MED15.

for MED15 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for MED15 antibody (pAb)References

 
MED15 antibody (pAb) tested by immunoprecipitation.

MED15 antibody (pAb) tested by Immunoprecipitation.
10 µl of MED15 antibody was used to immunoprecipitate MED15 from 400 µg of HeLa whole cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the MED15 antibody at a dilution of 1:1,000.

MED15 antibody (pAb) tested by Western blot.

MED15 antibody (pAb) tested by Western blot.
HeLa whole-cell extract (20 µg) probed with MED15 antibody at a dilution of 1:1,000.

for MED15 antibody (pAb)Background

MED15 (mediator of RNA polymerase II transcription subunit 15) is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway.

for MED15 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for MED15 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

MED15 antibody (pAb)
 

Data Thumbnails

MED15 antibody (pAb) tested by immunoprecipitation.

Immunoprecipitation of MED15 pAb.
(Click image to enlarge and see details.)

MED15 antibody (pAb) tested by Western blot.

Western blot of MED15 pAb.
(Click image to enlarge and see details.)