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NFATC2 antibody (pAb)

Aliases: NFAT1, NFATP
商品コード: 61621 Format: 100 µl ¥67,000 Buy Now
商品コード: 61622 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:120 kDa
Reactivity:Human

Applications

Western Blot Validated Immunoprecipitation Validated

for NFATC2 antibody (pAb)Application Notes


Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:2,000 dilution

for NFATC2 antibody (pAb)Immunogen

This antibody was raised against a peptide within the N-terminal region of human NFATC2.

for NFATC2 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for NFATC2 antibody (pAb)References

 
NFATC2 antibody (pAb) tested by immunoprecipitation.

NFATC2 antibody (pAb) tested by Immunoprecipitation.
10 µl of NFATC2 antibody was used to immunoprecipitate NFATC2 from 500 µg of Raji nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the NFATC2 antibody at a dilution of 1:500.

NFATC2 antibody (pAb) tested by Western blot.

NFATC2 antibody (pAb) tested by Western blot.
Detection of NFATC2 by Western blot analysis. NFATC2 was detected using a 1:500 dilution of NFATC2 (pAb) with 30 µg of Ramos nuclear extract.

for NFATC2 antibody (pAb)Background

NFATC2 (Nuclear Factor Of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2) plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway.

for NFATC2 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for NFATC2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

NFATC2 antibody (pAb)
 

Data Thumbnails

NFATC2 antibody (pAb) tested by immunoprecipitation.

Immunoprecipitation of NFATC2 pAb.
(Click image to enlarge and see details.)

NFATC2 antibody (pAb) tested by Western blot.

Western blot of NFATC2 pAb.
(Click image to enlarge and see details.)