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NRF1 antibody (pAb)

Aliases: ALPHA-PAL
商品コード: 61673 Format: 100 µl ¥67,000 Buy Now
商品コード: 61674 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:68 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated

for NRF1 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,500 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for NRF1 antibody (pAb)Immunogen

This antibody was raised against a peptide within the C-terminal region of human NRF1.

for NRF1 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for NRF1 antibody (pAb)References

 
NRF1 antibody (pAb) tested by Western blot.

NRF1 antibody (pAb) tested by Western blot.
Nuclear extract (30 µg) of C2C12 (Lane 1) and HepG2 cells (Lane 2) probed with NRF1 antibody at a 1:500 dilution.

for NRF1 antibody (pAb)Background

NRF1 (Nuclear Respiratory Factor 1) is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.

for NRF1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for NRF1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

NRF1 antibody (pAb)
 

Data Thumbnails

NRF1 antibody (pAb) tested by Western blot.

Western blot of NRF1 pAb.
(Click image to enlarge and see details.)