Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
抗体

Tet1 antibody (mAb)

Clone: 5D6
Aliases: LCX, Cxxc6, AA517754, BB001228, mKIAA1676, D10Ertd17e, 2510010B09Rik
商品コード: 61741 Format: 100 µg ¥67,000 Buy Now
商品コード: 61742 Format: 10 µg ¥19,000 Buy Now

Request a quote for a bulk orderBulk Order Button


Antibody Type:Monoclonal
Isotype:IgG2a
Purification:Protein G Chromatography
Host:Rat
Molecular Weight:215 kDa
Reactivity:Human, Mouse

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Immunocytochemistry Validated

for Tet1 antibody (mAb) (Clone 5D6)Application Notes


Validated Applications:
ChIP-Seq: 4 µl per ChIP

Published Applications:
ICC/IF
IP
WB
See reference for more information. Individual optimization may be required.

for Tet1 antibody (mAb) (Clone 5D6)Immunogen

This antibody was raised against a recombinant protein corresponding amino acids 1682-1914 of mouse Tet1.

for Tet1 antibody (mAb) (Clone 5D6)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

Also available as an AbFlex™ engineered recombinant antibody.  For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91171.

for Tet1 antibody (mAb) (Clone 5D6)References

 
Tet1 antibody (mAb) tested by ChIP-Seq.

TET1 antibody tested by ChIP-Seq.
ChIP was performed using TET1 antibody with 30 µg chromatin from human brain tumor cells and 4 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify TET1 binding sites. The image shows binding across a region of chromosome 1.

for Tet1 antibody (mAb) (Clone 5D6)Background

Tet1 (Ten-Eleven Translocation-1; methylcytosine dioxygenase) is a protein that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) which can lead to cytosine demethylation by either further oxidation via Tet proteins to 5-formylcytosine (5fC) and 5-carboxycytosine (5caC) or deamination into 5-hydroxymethyluracil (5hmU) and then subsequent replacement by unmethylated cytosine by the base excision repair system. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. Tet1 preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and polycomb-repressed genes. By controlling the levels of 5mC and 5hmC at gene promoters, it may regulate the gene expression silencing induced by cytosine methylation. May have a dual function by also repressing the expression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells, it plays a role in embryonic stem cells maintenance and inner cell mass cell specification.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Tet1 antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Tet1 antibody (mAb).

for Tet1 antibody (mAb) (Clone 5D6)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Tet1 antibody (mAb) (Clone 5D6)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Tet1 antibody (mAb)
 

Data Thumbnails

Tet1 antibody (mAb) tested by ChIP-Seq.

ChIP-Seq of Tet1 mAb.
(Click image to enlarge and see details.)