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Recombinant PRDM14 protein

Aliases: PFM11
商品コード: 31397 Format: 20 µg ¥79,000 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant PRDM14 proteinContents

Recombinant PRDM14 protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant PRDM14 proteinBackground

PRDM14 (PR Domain Containing 14) is a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. PRDM14 possesses histone methyltransferase activity. PRDM14 is a transcription factor that has both positive and negative roles on transcription. It is required for the maintenance of embryonic stem cell identity and the reacquisition of pluripotency in somatic cells. PRDM14 may play an essential role in germ cell development at two levels: the reacquisition of potential pluripotency, including Sox2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression. PRDM14 directly up-regulates the expression of pluripotency gene Oct4 through its proximal enhancer. PRDM14 binds to the DNA consensus sequence 5'-GGTC[TC]CTAA-3.'

for Recombinant PRDM14 proteinApplication Notes

Recombinant PRDM14 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

for Recombinant PRDM14 proteinProtein Details

Recombinant PRDM14 was expressed in a baculovirus expression system as the full length protein (accession number NP_004982.2) with an N-terminal FLAG-Tag. The molecular weight of PRDM14 is 67 kDa. The purity of the protein is > 80% by SDS-PAGE.

 
Recombinant PRDM14 protein gel.

Recombinant PRDM14 protein gel.
PRDM14 protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.

for Recombinant PRDM14 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant PRDM14 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant PRDM14 protein
 

Data Thumbnails

Recombinant PRDM14 protein gel.

Recombinant PRDM14 protein gel, stained with Coomassie Blue.
(Click image to enlarge and see details).