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リコンビナントタンパク質・酵素

Recombinant SIRT2 (50-356) protein

Aliases: SIRT-2, Sirtuin 2, SIR2-Like Protein, SIR2L, SIR2
商品コード: 31528 Format: 100 µg ¥73,000 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant SIRT2 (50-356) proteinContents

Recombinant SIRT2 (50-356) protein is supplied in 25 mM Tris pH 8.0, 150 mM NaCl, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant SIRT2 (50-356) proteinBackground

SIRT2 (Sirtuin 2) is a member of the SIR2 / Sirtuin family of NAD+-dependant deacetylase enzymes. It has multiple targets for deacetylation, including tubulin, p53, HOXA10, FOXO3A, P300 and FOXO1. SIRT2 suppresses adipogenesis by deacetylating FOXO1, promoting the binding of FOXO1 to PPARγ and subsequent repression of PPARγ mediated transcriptional activation. Similar to SIRT1, SIRT2 also deacetylates histone H4 at lysine 16. SIRT2 is present in the cytoplasm during most of the cell cycle except in the G2/M transition and in mitosis, where it is found in the nucleus, in contact with chromatin, and is required for exit from mitosis.

for Recombinant SIRT2 (50-356) proteinApplication Notes

Recombinant SIRT2 protein is useful for histone deacetylase assays and the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

HDAC Activity Assay Conditions: 3 µM histone peptide H3K9ac (1-21aa) was incubated with SIRT2 (50-356) in reaction buffer including 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM MgCl2, 1 mM KCl, 0.1 mg/ml BSA and 50 µM NAD+ for 30 min at 37°C. HTRF assay was used for activity detection.

for Recombinant SIRT2 (50-356) proteinProtein Details

Recombinant SIRT2 (50-356) protein that includes amino acids 50 - 356 of human SIRT2 protein (accession number NM_012237.3) was expressed in E. coli and contains an N-terminal GST-Tag with a molecular weight of 66.5 kDa. The purity of the protein is ≥ 85% by SDS-PAGE.

 
Recombinant SIRT2 (50-356) SDS-PAGE

Recombinant SIRT2 Protein on 12.5% SDS-PAGE with Coomassie Blue staining

<b>HTRF assay for SIRT2 (50-356) activity.</b>

HTRF assay for SIRT2 (50-356) activity.
3 µM histone peptide H3K9ac (1-21) was incubated with SIRT2 (50-356) in reaction buffer for 30 min at 37°C. Anti-H3K9me0 antibody was used to detect reaction products.

HTRF assay for SIRT2 (50-356) activity

HTRF assay for SIRT2 (50-356) activity.
3 µM histone peptide H3K9ac (1-21) was incubated with SIRT2 (50-356) in reaction buffer for 30 min at 37°C. Anti-H3K9me0 antibody was used to detect reaction products.

for Recombinant SIRT2 (50-356) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant SIRT2 (50-356) proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant SIRT2 (50-356) protein
 

Data Thumbnails

Recombinant SIRT2 (50-356) SDS-PAGE

Recombinant SIRT2 Protein 12.5% SDS-PAGE with Coomassie Blue staining.
MW: 66.5 kDa. Purity: ≥ 85%
(Click image to enlarge and see details).

<b>HTRF assay for SIRT2 (50-356) activity.</b>

3 µM histone peptide H3K9ac (1-21) was incubated with SIRT2 (50-356) in reaction buffer for 30 min at 37°C. Anti-H3K9me0 antibody was used to detect reaction products.

HTRF assay for SIRT2 (50-356) activity

3 µM histone peptide H3K9ac (1-21) was incubated with SIRT2 (50-356) in reaction buffer for 30 min at 37°C. Anti-H3K9me0 antibody was used to detect reaction products.
(Click image to enlarge and see details).