RbAp46/48 antibody (pAb)

for RbAp46/48 antibody (pAb)Background

RbAp46 (Retinoblastoma protein associated protein 46) and RbAp48 (Retinoblastoma protein associated protein 48) are highly homologous histone chaperones that play key roles in protein complexes that function to establish and maintain chromatin structure. RbAp46/48 are found in a number of protein complexes involved in chromatin remodeling (Mi-2β, NURF & NURD), transcriptional repression (Prc2, Sin3) and chromatin assembly (CAF1). Both RbAp46 and RbAp48 are specifically found in the catalytic cores of histone deacetylase (HDAC) complexes that promote transcriptional repression of target genes, including those repressed by the retinoblastoma tumor suppressor protein.

RbAp46 binds to and enhances the activity of the type B histone acetyltransferase HAT1, an enzyme that acetylates histone H4 specifically at its Lys5 and Lys12 residues prior to their incorporation into nucleosomes during replication.

RbAp48 is an evolutionarily conserved subunit of the chromatin assembly factor-1 (CAF-1) complex, where it associates with two other subunits, known as p150 and p60 in human cells. It is also reported that E2F-1 and RbAp48 are physically associated in the presence of Rb and histone deacetylase, suggesting that RbAp48 could be involved in transcriptional repression of E2F-responsive genes.

for RbAp46/48 antibody (pAb)Immunogen

This antibody was raised against recombinant human RbAp46 protein.

for RbAp46/48 antibody (pAb)Application Notes

This RbAp46/48 antibody has been validated for use in Western blotting (1:500 - 1:2,000 dilution) and chromatin IP (4 µl per ChIP).

for RbAp46/48 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide.

for RbAp46/48 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for RbAp46/48 antibody.

for RbAp46/48 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for RbAp46/48 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.