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Histone H3 trimethyl Lys9 antibody (mAb)
| Clone: 2AG-6F12-H4 |
| Catalog No: 39285 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39286 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | IF | IP |
|---|---|---|---|
| 1:250 - 1:1,000 | Not tested. ChIP-validated version available: 39161. |
1:20 - 1:100 | Yes |
for Histone H3 trimethyl Lys9 antibody (mAb) (Clone 2AG-6F12-H4)Background
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 9 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SuvH39H1 or G9a. This methylated lysine can be demethylated by histone demethylases as JMJD1A, LSD1 or JMJD2C. Methylation of this residue is mainly associated with transcriptional repression.
for Histone H3 trimethyl Lys9 antibody (mAb) (Clone 2AG-6F12-H4)Immunogen
Peptide including trimethyl-lysine 9 of human histone H3.
for Histone H3 trimethyl Lys9 antibody (mAb) (Clone 2AG-6F12-H4)Application Notes
This Histone H3 trimethyl Lys9 antibody (Clone 2AG-6F12-H4) (H3K9me3) has been validated for use in Western blotting (1:250 - 1:1,000 dilution) and immunofluorescence (1:20 - 1:100 dilution), and reported to work in immunoprecipitation.
This antibody has not been validated in ChIP, but ChIP-validated antibodies are available for this target: 39161.
for Histone H3 trimethyl Lys9 antibody (mAb) (Clone 2AG-6F12-H4)Buffer
Concentrated culture supernatant containing 30% glycerol, 0.035% sodium azide.
for Histone H3 trimethyl Lys9 antibody (mAb) (Clone 2AG-6F12-H4)Positive Control
Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H3 trimethyl Lys9 antibody (Clone 2AG-6F12-H4).
Bridging Antibody to Improve ChIP, MeDIP and IP
Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Histone H3 trimethyl Lys9 antibody (mAb).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3 trimethyl Lys9 antibody (mAb).
Histone H3 trimethyl Lys9 mAb (Clone 2AG-6F12-H4) tested by Western blot.
HeLa acid extract (10 µg per lane) probed with Histone H3 trimethyl Lys9 mAb (Clone 2AG-6F12-H4) (1:500 dilution).
Histone H3 trimethyl Lys9 mAb (Clone 2AG-6F12-H4) tested by immunofluorescence.
Staining of HeLa cells with Histone H3 trimethyl Lys9 mAb (Clone 2AG-6F12-H4) (1:250 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).
Histone H3 trimethyl Lys9 mAb (Clone 2AG-6F12-H4) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 trimethyl Lys9 mAb (Clone 2AG-6F12-H4) for trimethyl Lys9 of histone H3. Various methylated H3 proteins were spotted onto PVDF and probed with 39285 at 1:750. The amount of protein (picomoles) spotted is indicated next to each row.
Lane 1: monomethyl-Lys9 modified-lysine analog of histone H3.
Lane 2: dimethyl-Lys9 modified-lysine analog of histone H3.
Lane 3: trimethyl Lys9 modified-lysine analog of histone H3.
for Histone H3 trimethyl Lys9 antibody (mAb) (Clone 2AG-6F12-H4)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H3 trimethyl Lys9 antibody (mAb) (Clone 2AG-6F12-H4)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
