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CHD2 antibody (pAb)

Aliases: EEOC
商品コード: 39363 Format: 200 µl ¥67,000 Buy Now
商品コード: 39364 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:260 kDa
Reactivity:Human, Other (Wide Range)

Applications

Western Blot Validated

for CHD2 antibody (pAb)Application Notes


Validated Applications:
WB: 1:250 - 1:1,000 dilution

The addition of 0.1% Tween 20 in all blocking solutions may reduce background. Individual optimization may be required.

for CHD2 antibody (pAb)Immunogen

This CHD2 antibody (pAb) was raised against a peptide corresponding to the C-terminus of human CHD2.

for CHD2 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for CHD2 antibody (pAb)References

 
CHD2 antibody (pAb) tested by Western blot.

CHD2 pAb tested by Western blot.
Detection of CHD2 by Western blot analysis. Nuclear extract of HEK293 cells (25 µg) was probed with CHD2 pAb at a 1:250 dilution.

for CHD2 antibody (pAb)Background

CHD (Chromo-ATPase/Helicase-DNA-binding) proteins, which are ATP-dependent chromatin remodeling factors implicated in RNA polymerase II transcriptional regulation, are characterized by the presence of a double chromodomains (Chromatin Organization Modifier) and SNF2-related helicase/ATPase domains. The CHD1/CHD2 sub-family of CHD proteins also contain a C-terminal DNA binding domain that recognizes AT-rich sequences. CHD proteins alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. CHD2 is required for normal development of mouse embryos.

for CHD2 antibody (pAb)Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for CHD2 antibody.

for CHD2 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for CHD2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

CHD2 antibody (pAb)
 

Data Thumbnails

CHD2 antibody (pAb) tested by Western blot.

Western blot of CHD2 pAb.
(Click image to enlarge and see details.)