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Histone H4 monomethyl Lys20 antibody (mAb)
| Clone: 5E10-D8 |
| Catalog No: 39727 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 39728 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| 0.5 - 2 µg/ml | Not tested. ChIP-validated version available: 39175. |
1 µg/ml |
for Histone H4 monomethyl Lys20 antibody (mAb) (Clone 5E10-D8)Background
Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
Histone 4 lysine 20 (H4K20) can be mono-, di- or trimethylated by different histone methyltransferases such as NSD1 or ASH1. The methylation of this lysine is often associated with transcriptional repression. Monomethylation of histone H4 at lysine 20 is detected on the inactive X chromosome in female mammals and implicated in chromosome condensation.
for Histone H4 monomethyl Lys20 antibody (mAb) (Clone 5E10-D8)Immunogen
This antibody was raised against a synthetic peptide containing monomethyl Lys20 of Histone H4.
for Histone H4 monomethyl Lys20 antibody (mAb) (Clone 5E10-D8)Application Notes
This antibody has been validated for use in Western blotting (0.5 - 2 µg per ml) and immunofluorescence (1 µg/ml).
This antibody has not been validated in ChIP, but ChIP-validated antibodies are available for this target: 39175.
for Histone H4 monomethyl Lys20 antibody (mAb) (Clone 5E10-D8)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
for Histone H4 monomethyl Lys20 antibody (mAb) (Clone 5E10-D8)Positive Control
Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H4 monomethyl Lys20 mAb (Clone 5E10-D8).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H4 monomethyl Lys20 antibody (mAb).
Histone H4 monomethyl Lys20 mAb (Clone 5E10-D8) tested by Western blot.
Detection of Histone H4 monomethyl Lys20 by Western blot. The analysis was performed using nuclear extract of HeLa cells probed with Histone H4 monomethyl Lys20 pAb (Clone 5E10-D8) at a dilution of 1 µg/ml.
Histone H4 monomethyl Lys20 mAb (Clone 5E10-D8) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H4 monomethyl Lys20 mAb (Clone 5E10-D8) for monomethyl-lysine 20 of histone H4. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H4 monomethyl Lys20 mAb (Clone 5E10-D8) at 1 ug/ml. The amount of peptide (picomoles) spotted is indicated next to each row.
Top panel:
Lane 1: unmodified Lys79 peptide
Lane 2: monomethyl Lys79 H4
Lane 3: dimethyl Lys79 H4
Lane 4: trimethyl Lys79 H4
Lane 5: monomethyl K20 H4
Lane 6: dimethyl K20 H4
Lane 7: trimethyl K20 H4
Bottom panel:
Lane 1: monomethyl Lys31 H4 peptide
Lane 2: dimethyl Lys31 H4 peptide
Lane 3: trimethyl Lys31 H4 peptide
Lane 4: monomethyl Lys44 H4 peptide
Lane 5: dimethyl Lys44 H4 peptide
Lane 6: trimethyl Lys44 H4 peptide
Histone H4 monomethyl Lys20 antibody tested by immunofluorescence.
Detection of Histone H4 monomethyl Lys20 by immunofluorescence. HeLa cells were stained with Histone H4 monomethyl Lys20 antibody at a dilution of 1 µg/ml. Top panel: Histone H4 monomethyl Lys20 antibody staining. Middle panel: DAPI. Bottom panel: merge.
for Histone H4 monomethyl Lys20 antibody (mAb) (Clone 5E10-D8)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H4 monomethyl Lys20 antibody (mAb) (Clone 5E10-D8)Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
