Bub1 antibody (mAb)

for Bub1 antibody (mAb) (Clone 14H5)Background

Bub1 is a serine-threonine protein kinase and an important regulator of cell division. It is essential for the G2/M mitotic spindle assembly/integrity checkpoint as well as critical for chromosome alignment. Bub1 is required for the assembly of a large number of proteins at the kinetochore, including CENP-E, CENP-F, BUB1B, MAD2L1 and PLK1. It plays a role in sister chromatid cohesion and it serves as a substrate for the anaphase-promoting complex. Bub1 mediates cell death in response to chromosome mis-segregation and acts to suppress spontaneous tumorigenesis.

for Bub1 antibody (mAb) (Clone 14H5)Immunogen

This Bub1 antibody was raised against a recombinant protein corresponding to amino acids 281-419 of human Bub1.

for Bub1 antibody (mAb) (Clone 14H5)Application Notes

This Bub1 antibody was validated in Western blot (0.1 - 1.0 µg/µl dilution) and immunofluorescence (0.5 - 1.0 µg/µl dilution). For details on the usage of this antibody, please see the references below.

for Bub1 antibody (mAb) (Clone 14H5)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for Bub1 antibody (mAb) (Clone 14H5)References

Liu, ST et al, 2003. Nat Cell Biol 5: 341-345.
Liu, ST et al, 2006. J Cell Biol 175: 41-53.

for Bub1 antibody (mAb) (Clone 14H5)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Bub1 antibody (mAb) (Clone 14H5)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.