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Histone H3 antibody (mAb)

Clone: MABI 0301
商品コード: 39763 Format: 100 µg ¥67,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2b
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:17 kDa
Reactivity:Human, Other (Wide Range)

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for Histone H3 antibody (mAb) (Clone MABI 0301)Application Notes


Validated Applications:
ChIP-Seq: 4 µg per ChIP
ChIP: 5 - 10 µg per ChIP
ICC/IF: 1 µg/ml dilution
WB: 0.5 - 2 µg/ml dilution

Published Applications:
ChIP
ICC/IF
WB
Proximal Ligation Assay (PLA)

See references for more information. Individual optimization may be required.

for Histone H3 antibody (mAb) (Clone MABI 0301)Immunogen

This Histone H3 antibody (mAb) was raised against a peptide containing the N-terminus of histone H3.

for Histone H3 antibody (mAb) (Clone MABI 0301)Buffer

PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, a PBS only version of this antibody is also available.

for Histone H3 antibody (mAb) (Clone MABI 0301)References

 
Histone H3 antibody (mAb) tested by ChIP-Seq.

Histone H3 antibody (mAb) tested by ChIP-Seq.Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from Farage human lymph-node derived B cells and 4 µg Histone H3 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 17.7 million sequence tags were mapped to identify Histone H3 binding sites.

Histone H3 antibody (mAb) tested by ChIP.

Histone H3 antibody (mAb) tested by ChIP.
ChIP performed on Active Motif Ready-to-ChIP HeLa cell chromatin (Catalog No. 53015) using Histone H3 antibody (mAb). PCR was performed using primers specific for the promoter region of the human GAPDH gene.
Lane 1: ChIP using mouse IgG as a negative control.
Lane 2: ChIP using 10 µg of Histone H3 antibody (mAb).
Lane 3: Input DNA control.

Histone H3 antibody (mAb) tested by Immunofluorescence.

Histone H3 antibody (mAb) tested by immunofluorescence.
HeLa cells stained with Histone H3 antibody (mAb) at a dilution of 1 µg/ml.

Histone H3 antibody (mAb) tested by Western blot.

Histone H3 antibody (mAb) tested by Western blot.
HeLa nuclear extract (20 µg per lane) probed with Histone H3 antibody (mab) at a dilution of 2 µg/ml.
    Lane 1: Nuclear extract of untreated HeLa cells.
    Lane 2: Nuclear extract of HeLa cells treated with sodium butyrate.

for Histone H3 antibody (mAb) (Clone MABI 0301)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure.

Each histone contains two domains. First, a main globular domain (C-terminal) forming the core of the nucleosome is involved in histone-histone interactions and in binding to the DNA. Secondly, an N-terminal tail is subject to post-translational modifications. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

for Histone H3 antibody (mAb) (Clone MABI 0301)Positive Control

Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H3 antibody (Clone MABI 0301).

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3 antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Histone H3 antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3 antibody (mAb).

for Histone H3 antibody (mAb) (Clone MABI 0301)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3 antibody (mAb) (Clone MABI 0301)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

This antibody is manufactured by MAB Institute, Inc.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H3 antibody (mAb)
 

Data Thumbnails

Histone H3 antibody (mAb) tested by ChIP-Seq.

ChIP-Seq of Histone H3 mAb.
(Click image to enlarge and see details.)

Histone H3 antibody (mAb) tested by ChIP.

ChIP analysis of Histone H3 mAb.
(Click image to enlarge and see details.)

Histone H3 antibody (mAb) tested by Immunofluorescence.

Immunofluorescence (IF) staining with Histone H3 mAb.
(Click image to enlarge and see details.)

Histone H3 antibody (mAb) tested by Western blot.

Western blot of Histone H3 mAb.
(Click image to enlarge and see details.)