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SATB1 antibody (pAb)

Aliases: Special AT-Rich Sequence Binding Protein 1
商品コード: 39839 Format: 100 µl ¥67,000 Buy Now
商品コード: 39840 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:105 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated

for SATB1 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

for SATB1 antibody (pAb)Immunogen

This SATB1 antibody was raised against a peptide within the N-terminal region of SATB1.

for SATB1 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for SATB1 antibody (pAb)References

 
SATB1 antibody (pAb) tested by Western blot.

SATB1 antibody (pAb) tested by Western blot.
Whole cell extract of mouse thymus cells (30 µg per lane) probed with SATB1 antibody (pAb) (1:2,000 dilution).

for SATB1 antibody (pAb)Background

SATB1 (Special AT-rich sequence-binding protein 1) is a chromosome-associated protein that binds to matrix attachment regions (MARs) of DNA at special AT-rich sequences and induces local chromatin-loop remodeling. SATB1 functions as both a chromatin organizer and transcription factor. It regulates gene expression and higher-order chromatin structure of multigene clusters. SATB1 acts as a docking site for several chromatin remodeling enzymes and recruits corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. It is required for the switching of fetal globin genes, plays a role in chromatin organization and nuclear architecture during apoptosis. It is a crucial factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis. SATB1 contributes to metastatic potential in breast cancer cells, directly upregulating metastasis-associated genes while downregulating tumor-suppressor genes. It is also required for the differentiation of embryonic stem cells through repression of ES pluripotency factors Nanog, Klf4, and Tbx3.

for SATB1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for SATB1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

SATB1 antibody (pAb)
 

Data Thumbnails

SATB1 antibody (pAb) tested by Western blot.

Western blot of SATB1 pAb.
(Click image to enlarge and see details.)