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Nanog antibody (mAb)

Clone: 2E6E1
Aliases: ENK, ecat4, 2410002E02Rik
商品コード: 61627 Format: 100 µg ¥67,000 Buy Now
商品コード: 61628 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2a
Purification:Protein G Chromatography
Host:Rat
Molecular Weight:45 kDa
Reactivity:Mouse

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for Nanog antibody (mAb) (Clone 2E6E1)Application Notes


Validated Applications:
ICC/IF: 2 - 10 µg/ml dilution
WB: 1 - 2 µg/ml dilution

for Nanog antibody (mAb) (Clone 2E6E1)Immunogen

This antibody was raised against a peptide comprising amino acids 2-25 of mouse Nanog.

for Nanog antibody (mAb) (Clone 2E6E1)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Nanog antibody (mAb) (Clone 2E6E1)References

 
Nanog antibody (mAb) tested by immunofluorescence.

Nanog antibody (mAb) (Clone 2E6E1) tested by immunofluorescence.
Top: HeLa cells stained with Nanog antibody (mAb). Bottom: Hoechst staining.

Nanog antibody (mAb) tested by Western blot.

Nanog antibody (mAb) (Clone 2E6E1) tested by Western blot.
Nanog antibody detection by Western blot. The analysis was performed using 30 µg of mouse ES nuclear extract and Nanog antibody at a 2 µg/ml dilution.

for Nanog antibody (mAb) (Clone 2E6E1)Background

Nanog (Nanog homeobox) is a transcriptional regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation (By similarity).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Nanog antibody (mAb).

for Nanog antibody (mAb) (Clone 2E6E1)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Nanog antibody (mAb) (Clone 2E6E1)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Nanog antibody (mAb)
 

Data Thumbnails

Nanog antibody (mAb) tested by immunofluorescence.

Immunofluorescence stain of Nanog mAb.
(Click image to enlarge and see details.)

Nanog antibody (mAb) tested by Western blot.

Western blot of Nanog mAb.
(Click image to enlarge and see details.)